Inforamtion on cutting hard tissues

Hello everybody,

As part of ongoing research at our company I was trying to cut uniform cross
sections of bovine hooves across the horizontal plane. We recently bought a new
sliding microtome (HM 440E, Carl Zeiss, Inc.) and were using it with a
Tungsten-carbide knife.  Unfortunately the tissue is much harder than what we
expected and we were unsucessful. I was wondering if anyone out there had a
similar experience with this kind of material or with some other hard material.
I would be grateful if someone could give me tips about any how to cut hard
materials or inform me of any softening methods used for hard keratinous

I am new to the process of microtomy and also a new addition to this list.

Thanks for your time.

Sanjay Sharma

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