FW: ck 5/6

From:Jim Manavis <jim.manavis@imvs.sa.gov.au>


We use a Boehringer antibody to CK5/6. We initially microwave citrate
retrieve then post treat with trypsin to. Overnight incubation at 1/4000.
Works wonderfully well.

Jim Manavis
Senior Hospital Scientist
Department of Neuropathology
Institute of Medical & Veterinary Science
Adelaide, SA, 5000
Phone: 61-08-8222-3668
FAX: 61-08-8222 3204
e.mail: jim.manavis@imvs.sa.gov.au
Disclaimer: Not this little black duck!

-----Original Message-----
From:	Teri Johnson [mailto:terij@prlnet.com]
Sent:	Monday, 6 November 2000 7:54 pm
To:	histonet
Subject:	ck 5/6

Hi to all out there!
Is anyone currently doing ck 5/6 successfully?  A friend of mine is trying
to work this antibody up and isn't having any luck.  She's using Biocare
Medical's antibody with their suggested protocol and so far, nada.
Any help is very much appreciated!
-Teri Johnson
Supervisor, Anatomic Pathology
Physicians Reference Laboratory
Overland Park, KS

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