Antigen Retrieval Methods

From:Daniel Martinez <>

     I currently have a rare Parkinson's case that I
am having trouble staining.  This tissue has been
fixing in formalin for 13yrs.  I am attempting to
stain this tissue with several alpha-synuclein
antibodies that we produce.  To date, I have tried
formic acid, proteinase-K, microwave, and boiling
treatments.  Any advice on other methods that might be
worth trying would be appreciated.  Thanks for your

Dan Martinez
CNDR/University of Pennsylvania  

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