Antigen Retrieval Methods
From: | Daniel Martinez <nativenewjersian@yahoo.com> |
I currently have a rare Parkinson's case that I
am having trouble staining. This tissue has been
fixing in formalin for 13yrs. I am attempting to
stain this tissue with several alpha-synuclein
antibodies that we produce. To date, I have tried
formic acid, proteinase-K, microwave, and boiling
treatments. Any advice on other methods that might be
worth trying would be appreciated. Thanks for your
help
Dan Martinez
CNDR/University of Pennsylvania
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