waterbaths -Reply
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From: | "Richard Cartun" <Rcartun@harthosp.org> |
To: | <HistoNet@Pathology.swmed.edu>, <melodyricci@usa.net> |
Reply-To: | |
Date: | Wed, 19 May 1999 14:45:04 -0400 |
Content-Type: | text/plain; charset=US-ASCII |
I have been experimenting with a waterbath from Fisher Scientific for heat-induced epitope retrieval (HIER). The specific model number is "ISOTEMP 205". I use it primarily for the HercepTest, but it appears to work well for other antigens as well. I have it set at 95 degrees C. and place slides into the hot retrieval solution for a minimum of 20 minutes (40 minutes for HercepTest). The immunoreactivity on slides treated this way compares favorably with those treated with our Black & Decker "Flavor Scenter Steamers", but with less tissue destruction.
R. Cartun
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