mammary tissue frozens

<< Previous Message | Next Message >> (Neal Beeman)
Date:Thu, 20 May 1999 16:06:27 -0600
Content-Type:text/plain; charset="us-ascii"

I am cutting frozen sections of mouse mammary gland for immunofluorescence.
The mid lactation stages are pretty easy.  The pregnant stages however are
a greasy mess.  The gland accumalates a huge amount of fat during pregnancy
which is lost during lactation.  I am cutting the pregnant glands at the
lowest possible temperature(I put blocks of dry ice in the cryotome).  I
fix the gland in paraformaldehyde prior to freezing.  Does anyone know how
to make my block harder in a way that will not interfear with antigenicity?


<< Previous Message | Next Message >>