antigen retrieval using steamer
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From: | "Kathy Oprea" <koprea@calc.vet.uga.edu> |
To: | HistoNet@Pathology.swmed.edu |
Reply-To: | |
Date: | Tue, 18 May 1999 11:52:51 EST |
Content-Type: | text/plain; charset=US-ASCII |
Our lab has been routinely using the steamer for antigen retrieval
for 3 years with consistently good results. Our procedure is very
simple- We cut 4 micron sections for IHC. Fill steamer, with tap
water, and start. Deparaffinize slides in xylene, hydrate through
graded ETOH, to deionized water. Drain water from slides and place
them in citrate buffer. (We currently use BioGenex Antigen Retrieval
Citra, Catalog# HK086-9K. This comes in a 10X concentrate which we
dilute for a working solution, following manufacturers directions.)
Place slides/citrate buffer in steam chamber base. Cover
and steam for 20 minutes. Carefully remove covered steam chamber base
containing slides/citrate buffer, from steamer. Open the lid and let
slides/citrate buffer, remain inside steam chamber base for an
additional 5 mintes to cool. Proceed with routine IHC.
Feel free to contact me with questions.
Kathy
************ Thank you ************
* Kathy Oprea *
* University of Georgia *
* College of Veterinary Medicine *
* Athens, GA 30602-7388 *
* KOPREA@calc.vet.uga.edu *
The worker who's never said "Thank
God it's Friday," probably never
really worked.
************************************
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