Thioflavine T for amyloid
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From: | marilyn.johnson@agric.gov.ab.ca |
To: | histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Thu, 27 May 1999 08:03:04 -0600 |
Content-Type: | text/plain; charset=us-ascii |
The following procedure is from the Handbook of Histopathological and
Histochemical Techniques, Culling, third edition.
Fixation is not critical - used on paraffin or frozen sections.
Bring sections to water.
Stain in alum hematoxylin for 2 mins, to quench nuclear fluorescence - does
not have
to be differentiated, or blued.
Wash in water a few minutes.
Stain in 1% aqueous thioflavin T for 3 mins.
Rinse in water.
Differentiate in 1% acetic acid for 20 mins.
Wash in water.
Mount in Apathy's medium.
Amyloid will stain bright yellow (depending on the filter used).
Mast cell granules are yellow.
I found this worked very well for me - Good Luck !!
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