Re: macrophage IHC

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From:Jamie Erickson <JErickson@genetics.com>
To:HistoNet@Pathology.swmed.edu, jtsonger@vt.edu
Reply-To:
Date:Thu, 20 May 1999 17:37:53 -0400
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Jill,
     Mouse and monkeys Mac's are my thing mostly, and I have found a couple good one's. For monkey, rabbit, and human the HAM56 code # M0632 from DAKO works great on spleen and skin, nice staining of langerhan cells as well. These are formalin fixed paraffin embedded samples and trypsin or proteinase K digested.
     For mouse I use a BM8 antibody  vendor BACHEM cat # T-2006.  also formalin fixed paraffin embedded samples and trypsin or proteinase K digested.
For Rat I use a ED-1 or ED-2 antibody can't find the spec sheet so I can't help you any more then that. Have fun these cells look great stained especially the dendritic cells of the skin.

Jamie Erickson

>>> Jill Songer <jtsonger@vt.edu> 05/20 11:26 AM >>>
Hi netters,

Does anyone have a source for antibodies for macrophages in species OTHER
than human? Or does this even exist? I have tried Zymed and Dako without
luck. Any help would be appreciated. Thanks

**************************************************************************
Jill Songer HT (ASCP)
Veterinary Teaching Hospital
College of Veterinary  Medicine
Virginia Tech
Blacksburg, VA


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