We are currently using Boehringer Mannheim's Cytokeratin 5/6 for frozen and
FFPE specimens, primarily for suspected Mesotheliomas.  Antigen retrieval
is accomplished with Citrate Buffer.



Thomas E. Brooks
M. D. Anderson Cancer Center
Immunocytochemistry Lab - Box 85
1515 Holcombe Blvd.
Houston, TX 77030





"Murray HORN" <muza@globec.com.au> on 05/21/99 05:54:31 AM

To:   "Histonet" <HistoNet@Pathology.swmed.edu>
cc:    (bcc: Thomas E. Brooks/MDACC)
Subject:  cytokeratin 5/6




Hi all
Has anyone successfully used the cytokeratin 5/6 antibody on FFPE tissue.
If so what sort of retrieval was used

Thanks in anticipation

Murray Horn