Re: cleaning diamond knives
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From: | "R.Wadley" <s9803537@pop3.unsw.edu.au> |
To: | Histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Mon, 31 May 1999 08:42:24 +1000 |
Content-Type: | text/plain; charset="us-ascii" |
Dear Carla,
Sounds like a good way to clean the knives & save some $. Traditionally
pith from various reeds has been used, but more recently various companies
have been supplying polystyrene sticks. Appropriately trimmed packing
peanuts should be fine, (although I wonder if they might be a little firm),
provided you use all the usual care & attention. However I have always
used 100% ethanol when wiping the edge of the blade. Distilled water is
for those rare occassions when you need to soak the whole knife to remove
some particularly nasty adherent section. I section onto 20% ethanol to
remove wrinkles. This eliminates the need for those various noxious
substances usually waved over the top of the waterbath. I use Epon resin
&/or its substitutes, Spurrs may have a very low viscosity but I find it
too toxic for all but exceptional circumstances.
Hope this helps
Regards
Rob
At 07:47 AM 5/28/99 -0600, you wrote:
>Hi everyone
>I am wondering what the rest of you use to clean your diamond knives after
>sectioning (we use Spurr's resing). I have been told that packing peanuts
>soaked in distilled water work well. Hmmm. Looking forward to any other
ideas
>or comments.
>Thank you very much,
>Carla Aiwohi
>Western Fisheries Research Center
R. Wadley, B.App.Sc, M.L.S
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) +61 (2) 9385 3517
Ph (AH) +61 (2) 9555 1239
Fax +61 (2) 9385 1591
E-mail r.wadley@unsw.edu.au
www http://www.unsw.edu.au/clients/microbiology/CAF.html
(Under development)
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