Re: WHICH paraffin wax
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From: | "Sarah Christo" <schristo@cvm.tamu.edu> |
To: | <Allison@cardiff.ac.uk>, <HistoNet@Pathology.swmed.edu>, <jaa@rshhis.demon.co.uk> |
Reply-To: | |
Date: | Mon, 17 May 1999 16:05:20 -0500 |
Content-Type: | text/plain; charset=US-ASCII |
To Russ(ia, w/l),
When you said, "ps. I "used" to have a passion for many things once!!!" it made you sound like you are ready for the ole bone yard. Or is it to many slings and arrows on the Histonet. ;) Sarah
PS: Maybe a trip to Scotland is in order, get some of whatever Ian has been putting in his tea.
Sarah Christo, HT (ASCP)
Texas A&M University
College of Veterinary Medicine
Dept. of Vet. Anatomy & Public Health
College Station, TX 77868-4458
schristo@cvm.tamu.edu
>>> RUSS ALLISON <Allison@cardiff.ac.uk> 05/17 8:16 PM >>>
Jim,
I've said this many times before; extensive testing has convinced me
that there is very little difference between the waxes on the market
for histology.
We use plain, pure paraffin wax (which we happen to buy from Raymond
A Lamb). You just need to be assured that the melting point (so
called) is (roughly) what it purports to be and that the wax is
"clean". We use it for routine including teeth and other decalcified
tissue, FNAs and anything else.
I did have a passion for b-terpene enhanced wax (Hercules Chemical Co
- piccolyte), which was once marketed by a us supplier (Surgipath, I
believe) and an told Hercules has stopped producing that. There are
a variety of other beta-terpenes that can be used and my strong hunch
is that Colophonium resin (as recently featured on these pages) would
do.
Only problem with the terpene additives is that your floating out
bath needs to be 10 degrees or so lower.
It certainly enhances the "adhesion" between the tissue and wax block
following embedding.
However, all of that counts for nothing, as, microtomy and
microtomists being what they are, favourite waxes will always cut
better, thinner, quicker sections on a blade that lasts twice as
long!
Be interested to know the outcome of your survey.
Best wishes
Russ
ps. I "used" to have a passion for many things once!!!
Russ Allison, Wales
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