Re: Troubleshooting stains
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| From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
| To: | andreah@imclone.com, Histonet <Histonet@Pathology.swmed.edu> |
| Reply-To: | |
| Date: | Sun, 23 May 1999 14:25:03 -0400 (EDT) |
| Content-Type: | TEXT/PLAIN; charset=US-ASCII |
Andrea: There is a recent book that might help you with
your difficulty with Giemsa staining. Here's a re-posting
of a note I sent to the HistoNet lisserver a few months
ago.
> It is: Troubleshooting Histology Stains, by Richard W. Horobin
> and John D. Bancroft. New York and Edinburgh: Churchill Livingstone.
> pp. xvi + 266, with 36 colour plates. ISBN 0 443 05312 X. Needless
> to say, both authors are well known and are authors of other
> books in the field.
>
> A short introduction is followed by the coloured photos, which
> illustrate tissues correctly stained by several methods. The
> main part of the book contains accounts of 58 different staining
> methods, in alphabetical order. Each account begins with
> background information about how the method works. This is
> followed by hints on avoiding problems (What types of specimen
> processing are suitable? Is the procedure easy to carry out?)
> and on dealing with problems that do arise (e.g. Staining
> solution looks peculiar; Tissue stains unexpectedly
> weakly; Unexpected structures stain). Each type of difficulty
> is explained, with instructions for putting it right or avoiding
> it next time. There are references in the text, a 10-page
> bibliography, and also a list of references to books that give
> practical instructions for all the methods considered.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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