Re: Masson's Trichrome

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From:"J. A. Kiernan" <>
To:Brandon Schanbacher <>
Date:Wed, 26 May 1999 01:03:47 -0400 (EDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII

On Tue, 25 May 1999, Brandon Schanbacher wrote:

> I have tried to do a Masson's Trichrome stain for collagen on some
> sections of rat heart which are horribly overfixed. ...

> Is there any way to get better staining out of overfixed tissues? ...

   A simpler method, with not so many things to vary, should
   be the answer.  Picro-sirius red is an excellent collagen
   stain that works pretty well every time. It's an improvement
   on van Gieson, and will show very thin collagen fibres. The
   sensitivity is greatly enhanced if you have  polarizing optics
   on your microscope.  The method is in several textbooks.  If
   your library is lousy (so many are!), just ask again and I'll
   send you the detailed instructions.

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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