Jim,

I don't know anything about histo-freeze, per se, but I have done this
technique on blocks that have been hard to cut.  In my case, the ones that
were hard to cut were the ones that weren't fixed as well.  If your tissue
target is liable (likes to move if not fixed) it may diffuse more in the
tissue that wasn't fixed as well.  As this is the same tissue that would
require more of the histo-freeze, it would look as though the histo-freeze
caused the loss in label.

Just a thought.

Karen Pawlowski MS
Sr. Research Assoc.
UTSW Med. Ctr. Dallas/
PhD Candidate
UT Dallas, Dallas,TX

On Thu, 27 May 1999, Jim Ball wrote:

> 
> -----Original Message-----
> From: Jim Ball <xryhisto@ovis.net>
> To: Histonet@pathology.swmed.com <Histonet@pathology.swmed.com>
> Date: Wednesday, May 19, 1999 8:10 PM
> Subject: histofreeze
> 
> 
> >Has there ever been any research done on how histo-freeze affects IHC
> >prcedures, when it is sprayed directly on the face of a tissue block
> >(embeded in wax) in order to obtain a section. I personally detest the
> >stuff, but everyone in the deparment swears by the product. The only
> >evidence That I have that it might be effecting the IHC procedures is that
> >my S100 and HMB45 control block seemed to lose staining intensity the more
> >times it was exposed to the spray. There is no way the powers to be will
> >listen to any thing I say unless it comes from outside their little
> >universe. My main concern is that weak reactive sites may be lost
> completely
> >by even short exposures to histo-freeze.
> >
> 
> 
>