Re: Eosinophil granule stain

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From:"Histomail\\" <histomail@netspace.net.au>
To:"Ian Montgomery" <ian.montgomery@bio.gla.ac.uk>
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Date:Wed, 26 May 1999 09:52:10 +1000
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Dear Ian ,
any of the Romanowsky stains will work well on sections ie. Giemsa,
Leishman, or wrights but you will need a pH of 6.4  or even 6.0 rather than
the 6.8 Haematology use. Your routine H&E will also do extremely well if you
use a buffered eosin pH5.2-5.4 as will also EA50 from Cytology which has the
same pH. If you want a recipe for the Eosin Alc. or Aq. let me know.
Regards Mike (Downunder)
-----Original Message-----
From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
To: HistoNet@Pathology.swmed.edu <HistoNet@Pathology.swmed.edu>
Date: Tuesday, 25 May 1999 6:22
Subject: Eosinophil granule stain


>>Date: Mon, 24 May 1999 12:15 -0400 (EDT)
>>From: nelso019@mc.duke.edu
>>Subject: Eosinophil granule stain
>>To: histonet@pathology.swmed.edu
>>Mime-Version: 1.0
>>
>>     Does anyone know of a stain for eosinophils? I tried Luna's method
and
>>     the P.I. was not pleased. I understand there was one in the Journal
of
>>     Histotechnology a couple of years ago. Any suggesstions would be
>>     helpful.
>>
>>     Thanks in advance,
>>     Ramona Nelson
>>     DUMC Dept. of Pathology
>>     nelso019@mc.duke.edu
>>     919-684-6209
>>
>
>Ramona,
> Try this stain, reliable and gives good images.
>Ian.
>
>CARBOL CHROMOTROPE.  (Eosinophils.)
> Lendrum. 1944.  J.Path.Bact. 56.  441.
> Simple and selective technique for eosinophils. The presence of
>phenol lowers the pH of the stain increasing the selectivity for the
>eosinophilic granules.
>
>STAIN.
>  Phenol.  =  1 g.  (Melt in a flask under hot water.)
>  Chromotrope 2R.  =  0.5 g.  (Add to the melted phenol and mix.)
>  Distilled water.  =  100 ml.  (Dissolve the dye sludge.)
>  The stain keeps for up to 3 months.
>
>METHOD.
>1.) Bring sections to water.
>2.) Counterstain nuclei with Haemalum. (If required - looks good.)
>3.) Blue in running tap water.
>4.) Carbol Chromotrope.  =  30 mins.
>5.) Wash in running tap water.
>6.) D.C.M.
>
>RESULT.
> Eosinophil granules  =  Bright red.
> Nuclei.                    =  Blue/black.
>
>Dr. Ian Montgomery,
>West Medical Building,
>University of Glasgow,
>Glasgow,
>G12 8QQ,
>Scotland.
>Tel: 0141 339 8855 Extn. 6602.
>Fax: 0141 330 4100.
>e-mail: ian.montgomery@bio.gla.ac.uk
>
>
>
>




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