Re: Daily Digest

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From:Louise Burrell <lburrell@pathbox.wustl.edu>
To:HistoNet Server <HistoNet@Pathology.swmed.edu>
Reply-To:
Date:Wed, 19 May 1999 12:32:07 -0500 (CDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII

I put it in diet coke----tastes like the old lemon cokes of the fifties.

                                With warmest regards,
				Louise "Beezie" Burrell
				Chief Technologist-Cancer Center
				Tissue Procurement Core Facility
				Campus Box #8056
				Washington University School of Medicine
				St. Louis, MO.  63110
				Ph:  314-454-7615
				Fax: 314-454-5525

On Wed, 19 May 1999, HistoNet Server wrote:

> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 01:31:44 -0500
> From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
> Subject: Re: Pain FROM lab (Whither the vinegar?)
> 
> On Mon, 17 May 1999, Don Hammer wrote:
> 
> > The old home remedies may not be founded in science, but they usually
> > work! I luv vinegar and will do 2 tablespoons for the "pain in the neck"
> 
>   But what do you "do" with the vinegar? 
>  
>   -  Drink it - if so, what's the dose?
>   -  Rub it on your neck - if so, do you wait till it
>        evaporates or rub it off when it starts itching?
>   -  Put it in a heated bowl and inhale the fumes?
>   -  Shake it on some chips (= French fries) and eat them.
>   -  Place a "Vineg-Cure" granule ($12 each) under each
>        armpit and meditate about nothingness for an hour?
> 
>   I think we should be told. 
> 
>                              P.I.Fan.
> 
> - -------------------------------------------
> 
>                              
> 
>   
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 01:32:22 -0500
> From: DORIT ZHARHARY <d_zharhary@sigma.co.il>
> Subject: RE: water quality
> 
> It is better to use ultrapure water, however for in-situ hybridization (or any
> procedure where you do not need enzymatic activity) you do not have to treat
> the water with DEPC+ autoclave or autoclave any of your glassware, but instead
> add an RNase inhibitor to all solutions. This saves a lot of time. Such
> inhibitors, unlike those that are added where enzymatic activity is required,
> are relatively cheap, one example being a new product from Sigma called
> "protect-RNA".
> 
> Dorit
> 
> - ----------
> From:  Heike Grabsch
> Sent:  eai ude 17 iae 1999 04:53
> To:  histonet@Pathology.swmed.edu
> Subject:  water quality
> 
> A question for people who are doing studies with RNA like RT-PCR and in 
> situ hybridization for RNA:
> 
> what sort of water do you use?
> a) regular distilled water that is treated with DEPC and then autoclaved
> or 
> b) ultrapure water (f.ex. MilliQ)that is treated with DEPC and then 
> autoclaved
> 
> one of my collegues says that there is no need for ultrapure water, but I 
> would like to have some more opinions before I cut down my standards. (In 
> the lab where I was trained in molecular biology everything (also non-RNA 
> buffers etc) was prepared with ultrapure water.
> 
> thanks for some arguments
> 
> Dr. Heike Grabsch, Germany
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 03:30:10 -0500
> From: "I.H.Straatsburg DIVG" <I.H.Straatsburg@amc.uva.nl>
> Subject: LDH on paraffin liver sections
> 
> Has anyone tried staining lactate dehydrogenase (LDH) activity in 
> paraffin sections of formaldehyde-fixed liver?
> Most enzymes lose activity after fixation in 4% (w/v) PFA.
> Thanks in advance
> Irene Straatsburg
> ===========================================================
> Dr. I.H. Straatsburg
> IWO1-155, Dept. Exp. Surg., Surgical Lab., 
> AMC, Amsterdam, NL 1105 AZ
> tel. +31.20.5666653
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 06:15:55 -0500
> From: Tora Bardal <Tora.Bardal@chembio.ntnu.no>
> Subject: LR White?
> 
> Does anyone out there have any experience with different embedding media
> for immunohistochemistry at the LM and TEM level?
> 
> At present I use EPON for TEM and LM (sometimes Historesin for LM) and
> frozen sections for enzyme- and immunohistochemistry. One problem that
> arises with the latter technique is that material in the lumen of gut
> sections is washed away during the labelling procedure. I therefore want to
> try an alternative embedding medium, that infiltrates the tissue and keeps
> everything in place, and is suitable for immunohistochemistry AND
> examinations at the TEM level. I have heard that LR White is an
> alternative, but I do not have any experience with it. What are the
> advantages (or disadvantages) of this medium compared to others, such as
> Unicryl? I need information about polymerisation temperature, special
> equipment needed, special procedures before labelling, etc.
> 
> Thank you in advance for your help!
> 
> 
> 
> 
> ______________________________ 
>     .////.   .//       
>   o:::::::::///       
>  >::::::::::\\\      
>     '\\\\\'   \\  
> 
> Tora Bardal					tlf: + (47)73 59 09 38
> Department of Zoology, 			fax: + (47)73 59 63 11
> Norwegian University of Science and Technology (NTNU)
> Brattoera Research Center
> N-7491 Trondheim
> Norway
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 06:16:43 -0500
> From: "Rosebury, Wendy" <Wendy.Rosebury@wl.com>
> Subject: FW: Tissue Factor
> 
> I too am looking for an antibody for tissue factor but one that will work in
> dog and rabbit (hopefullly in formalin fixed, paraffin embedded.
> Thanks
> Wendy Rosebury
> Parke-Davis
> 
> 
> Hi, all!
> 
> I'm looking for antibody sources for Tissue Factor  --  staining in mouse
> tissue.  Thanks!
> 
> - -Patti Bourassa
>  Pfizer, Groton
> 
> 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 07:16:02 -0500
> From: Alex Brown <AlexB@nayrshire.scot.nhs.uk>
> Subject: RE: WHICH paraffin wax
> 
> Hi Jim,
> 	We routinely use Tissue Tek III wax from Bayer, with no bad
> experiences. However we recently tried a sample of Shandon's Histoplast
> which seemed equally as good. I would tend to side with Russ on this
> one, in that I don't think it makes a lot of difference. As long as it's
> 'clean' .
> 	Incidently, Russ  -  you're not that old !   (  yet :#194#)  )
> 		Regards,
> 			Alex Brown
> 			Crosshouse Hospital
> 			Kilmarnock, Scotland.
>  ----------
> From: Jim Almond
> To: histonet@Pathology.swmed.edu
> Subject: WHICH paraffin wax
> Date: 17 May 1999 16:17
> 
> I would value the opinion of UK histonetters (and further afield if the
> wax
> is marketed in the UK) regarding paraffin waxes for "routine" tissue
> embedding / microtomy. All experiences good or bad  would be
> appreciated.
> It would be interesting to see if there is a market leader or are we
> using
> a wide variety?
> In the absence of your comments, I could remain blissfully unaware of
> someones super product out there! The next issue is the thorny problem
> of
> whether to change?? Wax is one reagent to swear by, rather than swear
> at!!
> 
> Thanks in anticipation
> Jim
> Royal Shrewsbury Hospital, UK
> Royal Shrewsbury Hospitals NHS Trust
> Histology Dept
> Tel: +44 (0) 1743 261168  Fax: +44 (0) 1743 355963
> Email: hist@rshhis.demon.co.uk
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 07:29:35 -0500
> From: Clinomlabs@aol.com
> Subject: Fwd: Histotechnician Postion
> 
> 
>  
> 
> 
> 
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
> 
> 
> - ------------------ MIME Information follows ------------------
> 
> 
> - --part1_d4587e1e.2472b39b_boundary
> Content-Type: text/plain; charset="us-ascii"
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> 
> <<<<<< See above "Message Body (Could be empty)" >>>>>>
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> - --part1_d4587e1e.2472b39b_boundary
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> 
> Return-path: Clinomlabs@aol.com
> From: Clinomlabs@aol.com
> Full-name: Clinomlabs
> Message-ID: <d4587e1e.24718a95@aol.com>
> Date: Mon, 17 May 1999 11:07:01 EDT
> Subject: Histotechnician Postion
> To: HistoNet@Pathology.swmed.edu
> MIME-Version: 1.0
> Content-Type: text/plain; charset="us-ascii"
> Content-Transfer-Encoding: 7bit
> X-Mailer: AOL 4.0 for Windows 95 sub 13
> 
> Our laboratory (Clinomics, Inc.) located in Pittsfield, MA is currently 
> searching for a Histotechnician.  Responsibilities include maintaining 
> equipment and providing necessary monitoring and quality control/assurance.  
> Applicant must be proficient in basic histopathologic techniques, including 
> but not limited to fixation of tissue samples, tissue preparations in 
> paraffin, routine and specialized histology stains, immunohistochemistry 
> (IHC) and In situ hybridization (ISH).  Applicant may be required to 
> interface directly with clients and with all of our partner institutions.  
> Thus, the ability to communicate and develop positive relationships with ease 
> are essential.  Ability to work as a team member and learn new techniques is 
> essential.  The applicant must hold at least an Associate's degree in 
> Histotechnology or a related science.  Salary is based upon experience.  For 
> more information on our company visit our web site at www.clinomics.com or 
> contact Jeffrey Boehm-Laboratory Supervisor at (413) 447-1919 or e-mail at 
> clinomlabs@aol.com.
> 
> - --part1_d4587e1e.2472b39b_boundary--
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 07:30:06 -0500
> From: "LIZ STANDISH" <LSTANDISH@GOVSCI.COM>
> Subject: Re: Coverslip racks
> 
> Hello to all,
> 
> In regards to Catherine Johnson's request for cover glass staining racks.
> There are several different types which are available.  Shandon Lipshaw,
> Surgipath and EM science all have different versions, some are plastic, some
> are stainless steel. I am a vendor at Government Scientific Source. We are a
> small business distributor for all of the above mentioned companies and
> offer discount pricing on their products.  Please feel free to call me
> anytime and I will try to find your products at the best possible prices.
> 
> Liz Standish
> Government Scientific Source
> 800-248-8030
> Lstandish@GOVSCI.COM
> 
> - -----Original Message-----
> From: Catherine Johnson <johns373@maroon.tc.umn.edu>
> To: histonet@Pathology.swmed.edu <histonet@Pathology.swmed.edu>
> Date: Monday, May 17, 1999 6:06 PM
> Subject: Coverslip racks
> 
> 
> >Hi Histonetters,
> >
> >For a long time I've been wanting to get more coverslip racks for staining
> >(otherwise known as Chen boats), but I really didn't know where to get
> >them.  Finally, I found that Thomas Scientific carries them.  I was so
> >happy to find this out until I saw how much they cost-$89.95 each.  Why are
> >they soooo expensive?  Does any one know where to get them more
> inexpensively?
> >
> >Thanks for any feedback.
> >
> >Catherine Johnson
> >University of Minnesota
> >Neuromuscular Lab
> >
> >
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 08:31:20 -0500
> From: DORIT ZHARHARY <d_zharhary@sigma.co.il>
> Subject: RE: water quality
> 
> protect RNA is a new product. Catalog number R7397 (30 ml of 500X solution )
> 
> - ----------
> From:  Carpenter, Judith A.
> Sent:  eai uieue 18 iae 1999 13:44
> To:  'DORIT ZHARHARY'
> Subject:  RE: water quality
> 
> Dear Dorit-
> Would you have a catalog # for the "protect-RNA", all I can find in my
> Sigma catalog is something called "RNase ZAP" for cleaning glassware
> and plastics (cat#R2020).
> Thanks very much-
> Jude
> Jude Carpenter,BS, HTL(ASCP)
> Chief Technologist/Surgical Pathology/Histology/Autopsy
> FAHC/MCHV Campus
> 111 Colchester Ave.
> Burlington,   VT  05401
> (802)656-5116
> FAX : (802)656-3509
> jude.carpenter@vtmednet.org
> 
> 
> > -----Original Message-----
> > From:	DORIT ZHARHARY [SMTP:d_zharhary@sigma.co.il]
> > Sent:	Tuesday, May 18, 1999 3:17 AM
> > To:	histonet@Pathology.swmed.edu; 'Heike Grabsch'
> > Subject:	RE: water quality
> > 
> > It is better to use ultrapure water, however for in-situ hybridization (or
> > any procedure where you do not need enzymatic activity) you do not have to
> > treat the water with DEPC+ autoclave or autoclave any of your glassware,
> > but instead add an RNase inhibitor to all solutions. This saves a lot of
> > time. Such inhibitors, unlike those that are added where enzymatic
> > activity is required, are relatively cheap, one example being a new
> > product from Sigma called "protect-RNA".
> > 
> > Dorit
> > 
> > ----------
> > From:  Heike Grabsch
> > Sent:  eai ude 17 iae 1999 04:53
> > To:  histonet@Pathology.swmed.edu
> > Subject:  water quality
> > 
> > A question for people who are doing studies with RNA like RT-PCR and in 
> > situ hybridization for RNA:
> > 
> > what sort of water do you use?
> > a) regular distilled water that is treated with DEPC and then autoclaved
> > or 
> > b) ultrapure water (f.ex. MilliQ)that is treated with DEPC and then 
> > autoclaved
> > 
> > one of my collegues says that there is no need for ultrapure water, but I 
> > would like to have some more opinions before I cut down my standards. (In 
> > the lab where I was trained in molecular biology everything (also non-RNA 
> > buffers etc) was prepared with ultrapure water.
> > 
> > thanks for some arguments
> > 
> > Dr. Heike Grabsch, Germany
> > 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 08:31:49 -0500
> From: "LIZ STANDISH" <LSTANDISH@GOVSCI.COM>
> Subject: Fw: Coverslip racks
> 
> - ----Original Message-----
> From: LIZ STANDISH <LSTANDISH@GOVSCI.COM>
> To: Catherine Johnson <johns373@maroon.tc.umn.edu>;
> histonet@Pathology.swmed.edu <histonet@Pathology.swmed.edu>
> Date: Tuesday, May 18, 1999 8:58 AM
> Subject: Re: Coverslip racks
> 
> 
> >Hello to all,
> >
> >In regards to Catherine Johnson's request for cover glass staining racks.
> >There are several different types which are available.  Shandon Lipshaw,
> >Surgipath and EM science all have different versions, some are plastic,
> some
> >are stainless steel. I am a vendor at Government Scientific Source. We are
> a
> >small business distributor for all of the above mentioned companies and
> >offer discount pricing on their products.  Please feel free to call me
> >anytime and I will try to find your products at the best possible prices.
> >
> >Liz Standish
> >Government Scientific Source
> >800-248-8030
> >Lstandish@GOVSCI.COM
> >
> >-----Original Message-----
> >From: Catherine Johnson <johns373@maroon.tc.umn.edu>
> >To: histonet@Pathology.swmed.edu <histonet@Pathology.swmed.edu>
> >Date: Monday, May 17, 1999 6:06 PM
> >Subject: Coverslip racks
> >
> >
> >>Hi Histonetters,
> >>
> >>For a long time I've been wanting to get more coverslip racks for staining
> >>(otherwise known as Chen boats), but I really didn't know where to get
> >>them.  Finally, I found that Thomas Scientific carries them.  I was so
> >>happy to find this out until I saw how much they cost-$89.95 each.  Why
> are
> >>they soooo expensive?  Does any one know where to get them more
> >inexpensively?
> >>
> >>Thanks for any feedback.
> >>
> >>Catherine Johnson
> >>University of Minnesota
> >>Neuromuscular Lab
> >>
> >>
> >
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 09:00:34 -0500
> From: "Bashir, Naaz" <Naaz.Bashir@nrc.ca>
> Subject: Migration Indication
> 
> 
> The mail you sent to Bashir, Naaz has been forwarded to their new Exchange
> address. Their new address is Bashir, Naaz in addresslist Ottawa/Recipients,
> or NRC/OTTAWA/BASHIRN if you are using MS Mail.
> 
> Please update your Personal Address Book. If you have a problem doing this
> please contact your administrator.
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 09:14:29 -0500
> From: Dana Settembre <settembr@UMDNJ.EDU>
> Subject: Re-using
> 
> Does anyone re-use citra buffer  and H2O2 for surgical specimens?
> If the answer is yes, how long for each one?
> 
> Dana Settembre
> Immunohistochemistry Lab
> University Hospital
> Newark, NJ
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 09:28:31 -0500
> From: Margaret Blount <Margaret.Blount@unilever.com>
> Subject: Ag Retrieval
> 
> 
>           I too would be grateful for any guidelines, we at present 
>           use a pressure cooker but owing to local safety regulations 
>           we have to throw out a perfectly good pressure cooker and 
>           replace it with a new one every year! Because it is cheaper 
>           than having it tested, in case you wanted to know. I imagine 
>           that this would not be the case with a veggie steamer as 
>           pressure is not involved. The other issue is this, are the 
>           results as consistent as with a pressure cooker?
>           
>           Margaret Blount
>           Unilever Research
>           Bedford,
>           UK
>           Margaret.blount@unilever.com
> 
> 
> ______________________________ Forward Header
> __________________________________
> Subject: Ag Retrieval
> Author:  melodyricci@usa.net at INTERNET
> Date:    17/05/1999 21:31
> 
> 
> I have always done antigen retrieval methods using the microwave, and I have 
> read about those who use the veggie steamers.  Has anyone out there done 
> antigen retrieval in a waterbath?  Would they be willing to share a protocol?
>           
> Thanks!
>           
> M.Ricci
>           
> ____________________________________________________________________ 
> Get free e-mail and a permanent address at http://www.amexmail.com/?A=1
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 09:44:31 -0500
> From: "Connolly, Brett" <brett_connolly@merck.com>
> Subject: RE: Re-using
> 
> My understanding is that AR buffers are not reusable after heating as the pH
> is altered.
> 
> Brett
> 
> 
> Brett M. Connolly, Ph.D.
> Senior Research Biologist
> Dept. of Human Genetics
> Merck Research Laboratories
> WP26A-3000
> PO Box 4
> West Point, PA 19486
> tel. 215-652-2501
> FAX 215-652-2075
> 
> 
> > ----------
> > From: 	Dana Settembre[SMTP:settembr@UMDNJ.EDU]
> > Sent: 	Tuesday, May 18, 1999 10:10 AM
> > To: 	Histonet
> > Subject: 	Re-using
> > 
> > Does anyone re-use citra buffer  and H2O2 for surgical specimens?
> > If the answer is yes, how long for each one?
> > 
> > Dana Settembre
> > Immunohistochemistry Lab
> > University Hospital
> > Newark, NJ
> > 
> > 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 10:30:27 -0500
> From: "Terrett, Barb" <Barb_Terrett@pmh.toronto.on.ca>
> Subject: novored
> 
> Hi out there in histo land!!
> I was wondering if anyone has experience with a produst called novored from
> zymed. It is a red chromogen for immuno stained slides that withstands
> solvents. We have done a test run with a sample, and had very good results.
> Has anyone else used it, and if so, were there any Ab's it did not work well
> for and/or did you find it to be more sensitive than what you are using now?
> We are currently using AEC.
> Thanks very much
> b
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 10:53:16 -0500
> From: "OBR- Histo Lab" <histo@carolinas.org>
> Subject: 
> 
> 
> 
> - --------------------------------------------------------
> Name: histo
> E-mail: histo <histo@carolinas.org>
> Date: 05/18/99
> Time: 11:36:23
> 
> This message was sent by Z-Mail Pro - from NetManage
> NetManage - delivers Standards Based IntraNet Solutions
> - --------------------------------------------------------
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 11:20:14 -0500
> From: "Kathy Oprea" <koprea@calc.vet.uga.edu>
> Subject: antigen retrieval using steamer
> 
> Our lab has been routinely using the steamer for antigen retrieval 
> for 3 years with consistently good results. Our procedure is very 
> simple- We cut 4 micron sections for IHC. Fill steamer, with tap 
> water, and start. Deparaffinize slides in xylene, hydrate through 
> graded ETOH, to deionized water. Drain water from slides and place 
> them in citrate buffer. (We currently use BioGenex Antigen Retrieval 
> Citra, Catalog# HK086-9K. This comes in a 10X concentrate which we 
> dilute for a working solution,  following manufacturers directions.) 
> Place slides/citrate buffer in steam chamber base. Cover 
> and steam for 20 minutes. Carefully remove covered steam chamber base 
> containing slides/citrate buffer, from steamer. Open the lid and let 
> slides/citrate buffer, remain inside steam chamber base for an 
> additional 5 mintes to cool. Proceed with routine IHC.   
> Feel free to contact me with questions.
> Kathy 
> ************ Thank you ************
> * Kathy Oprea                     *
> * University of Georgia           *
> * College of Veterinary Medicine  *
> * Athens, GA 30602-7388           *
> * KOPREA@calc.vet.uga.edu         *
> The worker who's never said "Thank
> God it's Friday," probably never
> really worked.
> ************************************
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 11:20:50 -0500
> From: "OBR- Histo Lab" <histo@carolinas.org>
> Subject: Antibody Search
> 
> An all out call for help to anyone listening!! Our research 
> department is eager to find sources for the following 
> antibodies: GM3(ganglio);and one that differentiates between 
> male and female chromosomes.  We'd like to be able to use them 
> on formalin-fixed, paraffin-embedded tissue.  Any help is 
> greatly appreciated.
> - --------------------------------------------------------
> Name: histo
> E-mail: histo <histo@carolinas.org>
> Date: 05/18/99
> Time: 11:42:32
> 
> This message was sent by Z-Mail Pro - from NetManage
> NetManage - delivers Standards Based IntraNet Solutions
> - --------------------------------------------------------
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 11:21:22 -0500
> From: "Terrett, Barb" <Barb_Terrett@pmh.toronto.on.ca>
> Subject: BIG CORRECTION-NOVARED FROM VECTOR
> 
> Hi there histonutters,
> I owe the people at vector labs. a HUGE apology for misrepresenting their
> product as something from zymed. (Actually I should also apologize to the
> folks at zymed who are probably scratching their heads right now trying to
> figure out what I was talking about.) The product I am inquiring about is in
> fact NOVARED from VECTOR. As I said before, we had very promising results
> from this VECTOR product, and I am interested in anyone elses experience
> with this VECTOR product. 
> Again, I apologize for my error. Next time I will check the label instead of
> relying on my own feeble memory (all that formalin over the years!).
> Thanks for your help, I am looking forward to hearing more about NOVARED
> from VECTOR.
> ttfn b   ;)
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 11:21:48 -0500
> From: Judy Trogadis <judy@playfair.utoronto.ca>
> Subject: immuno sensitivity
> 
> Histonetters,
> 
> I am trying to detect a protein in brain tissue using immunocytochemistry.
> From northern and western blots we know that the RNA is made and is 
> translated into protein, now we want a more precise localization - it should
> be nuclear but we are not sure in which cell type(s) it is expressed.
> 
> I have used a biotinylated IgG followed by the ABC kit from Vector which is 
> a peroxidase reaction using DAB. No protein was detected although grossly,
> the tissue had more of a yellowish tint compared to negative controls. Also,
> we have a positive control which uses the same tissue preparation, i.e.
> fixation, cryosectioning, etc. One current theory is that the protein is      
> present in many cell types but at a low level of expression.
> 
> What would you say is the most sensitive immuno-detection method available?
> Would fluorescence be better?
> 
> Thanks in advance
> judy
> 
> 
> Judy Trogadis
> Eye Research Institute and
> University of Toronto
> Toronto Hospital, Western Div.
> 399 Bathurst  St.
> Toronto, Canada M5T 2S8
> 
> phone: 416-603-5088
> Fax:   416-603-5126
> email: judy@playfair.utoronto.ca
> 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 11:22:17 -0500
> From: "Carson, Karla" <KCarson@chw.edu>
> Subject: Ultrum II
> 
> Is anyone using the formalin substitute Ultrum II?  If so how has it
> affected immunostaining?
> 
> Karla Carson HT/HTL(ASCP)
> Regional Pathology Supervisor	
> Mercy Healthcare Sacramento
> 916-453-4494
> kcarson@chw.edu <mailto:kcarson@chw.edu> 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 12:59:38 -0500
> From: ALLISON@cardiff.ac.uk
> Subject: Competence
> 
> I know a lot of people got turned off by the recent Qualifications 
> debate and I know a lot of people get turned off by the bye-line at 
> the bottom of this message!
> Undaunted, here goes.
> 
> What do you do in the states about "competence"?
> Do you ever get tested on that?
> Do you have to prove you have retained it periodically?
> If yes to either or both, who tests it?
> Is it a condition of continuing to work (includes Florida and other 
> licensing states)?
> If so, how do you measure it?
> If not, should you have it?
> All comments very welcome in private or public.
> 
> I look forward to being swamped by your responses.
> 
> Many TIA
> Russ Allison, Wales
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 13:00:48 -0500
> From: Gwin Kerce <gkerce@arches.uga.edu>
> Subject: processing cost servay
> 
> 
> Help! Help!
> I have done a cost analysis for my lab using a reference from Tech
> Sample ASCP ( Barbara Davies, pub.1996).I need to compare cost with
> other institutions for a grant proposal. All replies will be
> confidential.
> 
> Please answer the following questions:
>    Reagents                            Cost and quantity of
> reagents?              Quantity need to fill processor?
> 10% Formalin
> 
> 100% ETOH
> 
> 95% ETOH
> 
> Xylene
> 
> Paraffin
> 
> What is the average number of  blocks run through the processor between
> reagent changes?
> 
> 
> What is the cost of your processor service contract?
> 
> 
> How many blocks can you embed in 1K (1.8L) of paraffin?
> 
> 
> Thank you,
> Gwin Kerce
> UGA
> Dept. Avian Medicine PDRC
> Athens GA 30602
> ph# 706-542-7262
> fax# 706-542-5630
> 
> 
> 
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
> 
> 
> - ------------------ MIME Information follows ------------------
> 
> 
> - --------------D093696CFD5C914F7BF0E48A
> Content-Type: text/plain; charset=us-ascii
> Content-Transfer-Encoding: 7bit
> 
> <<<<<< See above "Message Body" >>>>>>
> 
> - --------------D093696CFD5C914F7BF0E48A
> Content-Type: text/html; charset=us-ascii
> Content-Transfer-Encoding: 7bit
> 
> <!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
> <HTML>
> Help! Help!
> <BR>I have done a cost analysis for my lab using a reference from Tech
> Sample ASCP ( Barbara Davies, pub.1996).I need to compare cost with other
> institutions for a grant proposal. All replies will be confidential.
> <P>Please answer the following questions:
> <BR>  
> <U>Reagents </U>                          
> <U>Cost and quantity of
> reagents?</U>             <U>
> Quantity need to fill processor?</U>
> <BR>10% Formalin
> <P>100% ETOH
> <P>95% ETOH
> <P>Xylene
> <P>Paraffin
> <P>What is the average number of  blocks run through the processor
> between reagent changes?
> <BR> 
> <P>What is the cost of your processor service contract?
> <BR> 
> <P>How many blocks can you embed in 1K (1.8L) of paraffin?
> <BR> 
> <P>Thank you,
> <BR>Gwin Kerce
> <BR>UGA
> <BR>Dept. Avian Medicine PDRC
> <BR>Athens GA 30602
> <BR>ph# 706-542-7262
> <BR>fax# 706-542-5630</HTML>
> 
> - --------------D093696CFD5C914F7BF0E48A--
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 13:02:30 -0500
> From: Brandon Schanbacher <schanbacher.2@osu.edu>
> Subject: DAB film
> 
> Hello all,
> 
> We have had some problems with DAB solution leaving a light brown haze
> across the entire slide during immunostaining.  If anyone has any
> suggestions on how to remedy this situation, I'd appreciate any input.
> 
> The haze is most apparent when we use Upstate Biotechnologies' anti
> 3-nitrotyrosine antibody.  As far as our staining procedure, we use the
> Microprobe capillary gap system, which made us think it was a problem
> rinsing one of the antibody steps off of the slide.  We have increased
> the buffer rinses to no avail.  We don't get this film using other
> antibodies run at the same time, in the same DAB solution, so I doubt
> it's a problem with the DAB solution.  Has anyone else run into a
> similar situation?
> 
> 
> Thanks,
> 
> Brandon Schanbacher
> - --
> Division of Pharmacology
> Ohio State University
> 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 14:47:15 -0500
> From: "Connolly, Brett" <brett_connolly@merck.com>
> Subject: RE: immuno sensitivity
> 
> Judy,
> 
> Without a doubt you should try tyramide signal amplification (TSA). This is
> a perfect example of what this technology was developed for. You can use
> fluorescent or chromagenic detection. Check out the NEN website for details
> 
> http://www.nenlifesci.com/ti_link1.htm
> 
> Brett
> 
> 
> Brett M. Connolly, Ph.D.
> Senior Research Biologist
> Dept. of Human Genetics
> Merck Research Laboratories
> WP26A-3000
> PO Box 4
> West Point, PA 19486
> tel. 215-652-2501
> FAX 215-652-2075
> 
> > ----------
> > From: 	Judy Trogadis[SMTP:judy@playfair.utoronto.ca]
> > Sent: 	Tuesday, May 18, 1999 11:59 AM
> > To: 	HistoNet@Pathology.swmed.edu
> > Subject: 	immuno sensitivity
> > 
> > Histonetters,
> > 
> > I am trying to detect a protein in brain tissue using immunocytochemistry.
> > From northern and western blots we know that the RNA is made and is 
> > translated into protein, now we want a more precise localization - it
> > should
> > be nuclear but we are not sure in which cell type(s) it is expressed.
> > 
> > I have used a biotinylated IgG followed by the ABC kit from Vector which
> > is 
> > a peroxidase reaction using DAB. No protein was detected although grossly,
> > the tissue had more of a yellowish tint compared to negative controls.
> > Also,
> > we have a positive control which uses the same tissue preparation, i.e.
> > fixation, cryosectioning, etc. One current theory is that the protein is
> > 
> > present in many cell types but at a low level of expression.
> > 
> > What would you say is the most sensitive immuno-detection method
> > available?
> > Would fluorescence be better?
> > 
> > Thanks in advance
> > judy
> > 
> > 
> > Judy Trogadis
> > Eye Research Institute and
> > University of Toronto
> > Toronto Hospital, Western Div.
> > 399 Bathurst  St.
> > Toronto, Canada M5T 2S8
> > 
> > phone: 416-603-5088
> > Fax:   416-603-5126
> > email: judy@playfair.utoronto.ca
> > 
> > 
> > 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 14:48:00 -0500
> From: "Johnson, Jennifer(Hist)" <jjohnson3@genzyme.com>
> Subject: RE: Ag Retrieval
> 
> Hi Melody,
> I perform my antigen retrival in a plastic jar in a 90-99?C  waterbath.  I
> use the solution from Vector called "Antigen Unmasking Solution" diluted
> 1:100 in water.  I bring the solution to temperature, drop the slides in for
> 5-15 minutes (depending on the antibody), then remove the jar of slides from
> the waterbath and let that sit out on the bench for 15 minutes to cool down.
> Then I move on to the block or enzyme digestion or whatever step is next
> required.  It works like a charm on several of our collagen antibodies in
> tough tissues like bone and cartilage.   
> Please contact me offline if you want any more info!
> Jennifer Johnson
> jjohnson3gGenzyme.com
> > ----------
> > From: 	Melody Ricci
> > Sent: 	Monday, May 17, 1999 3:03 PM
> > To: 	Histonet Mailing List
> > Subject: 	Ag Retrieval
> > 
> > I have always done antigen retrieval methods using the microwave, and I
> > have
> > read about those who use the veggie steamers.  Has anyone out there done
> > antigen retrieval in a waterbath?  Would they be willing to share a
> > protocol?
> > 
> > Thanks!
> > 
> > M.Ricci
> > 
> > ____________________________________________________________________
> > Get free e-mail and a permanent address at http://www.amexmail.com/?A=1
> > 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 14:48:30 -0500
> From: "Hawkins, Hal" <hhawkins@SBI.UTMB.EDU>
> Subject: RE: LR White?
> 
> 
> LR White is useful for light microscopic immunolabeling, and
> very easy to work with.  We have found that gold labeling with
> silver enhancement gives a very pleasing high-resolution result
> at the light microscopic level.  Embedding only requires
> dehydration through alcohols and heating overnight in an oven
> at 55 or 60 degrees C.  It is necessary to exclude oxygen,
> however, so completely filled gelatin capsules are usually
> used.  Polyethylene embedding molds don't work because they
> allow oxygen to penetrate.  Sections can be cut with a glass
> knife.  Probably small blocks could be cut on a histologic
> microtome.  We got some Unicryl, but we didn't do a careful
> comparison.
> 
> Incidentally, a good solution to the problem of tissue loss
> from frozen sections is the Cryostat Frozen Sectioning Aid
> from Instrumedics, which glues the section tightly to the slide.
> We use one for frozens of skin of burn patients, and love it.
> 
> Hal Hawkins
> UTMB Galveston
> 
> 
> 
>  ----------
> From:  Tora Bardal
> Sent:  Tuesday, May 18, 1999 6:25 AM
> To:  histonet
> Subject:  LR White?
> 
> Does anyone out there have any experience with different embedding media
> for immunohistochemistry at the LM and TEM level?
> 
> At present I use EPON for TEM and LM (sometimes Historesin for LM) and
> frozen sections for enzyme- and immunohistochemistry. One problem that
> arises with the latter technique is that material in the lumen of gut
> sections is washed away during the labelling procedure. I therefore want   
> to
> try an alternative embedding medium, that infiltrates the tissue and   
> keeps
> everything in place, and is suitable for immunohistochemistry AND
> examinations at the TEM level. I have heard that LR White is an
> alternative, but I do not have any experience with it. What are the
> advantages (or disadvantages) of this medium compared to others, such as
> Unicryl? I need information about polymerisation temperature, special
> equipment needed, special procedures before labelling, etc.
> 
> Thank you in advance for your help!
> 
> 
> 
> 
> ______________________________
>     .////.   .//
>   o:::::::::///
>  >::::::::::\\\
>     '\\\\\'   \\
> 
> Tora Bardal     tlf: + (47)73 59 09 38
> Department of Zoology,    fax: + (47)73 59 63 11
> Norwegian University of Science and Technology (NTNU)
> Brattoera Research Center
> N-7491 Trondheim
> Norway
> 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 14:48:53 -0500
> From: Anita Jennings <jennings@mayo.edu>
> Subject: RE: water quality
> 
> We've used both nanopure and tap distilled for our RT-PCR. Does't seem to
> make much of a difference. anita
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 14:49:28 -0500
> From: "Coskran, Timothy M" <timothy_m_coskran@groton.pfizer.com>
> Subject: IHC on frozen sections
> 
> Hello,
> 
> Lately, I've had a problem with immuno on frozen sections.  There is a great
> deal of background staining showing up on my negative control. I'm not sure
> why this is happening since we use the same secondary antibodies and labels
> for paraffin and don't see this problem.  The problem is occurring with both
> DAB and Histomark red.  Any ideas?
> 
> Tim Coskran
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 14:50:06 -0500
> From: Melody Ricci <melodyricci@usa.net>
> Subject: waterbaths
> 
> Thanks for all the protocols on waterbath antigen retrieval.
> 
> Speaking of waterbaths, I'm currently shopping for a new one.  Our Shandon
> died and they no longer carry the computer board for the digital temperature
> settings.  Does anyone recommend any other brand of waterbaths that display
> the digital temperature?
> 
> Thanks for the help!
> 
> M.Ricci
> 
> 
> ____________________________________________________________________
> Get free e-mail and a permanent address at http://www.amexmail.com/?A=1
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 14:51:01 -0500
> From: "Instrumedics, Inc." <info@instrumedics.com>
> Subject: Frozen biopsies
> 
> The question of how to embed frozen tissue without melting is asked  from
> time to
> time. Noelle raised it with reference to a biopsy she received that was
> frozen in a centrifuge tube.
> 
> Those who have the Instrumedics Oil Accessory Kit can embed the frozen
> tissue in an oil, which is stored in an oil bath inside the cryostat. The
> oil  is at -8deg. C  and is liquid. The oil only freezes at -10deg C.
> 
> The cold  oil can be transferred to a chilled mold and the frozen tissue
> oriented in the liquid oil. The tissue will not melt!  The mold is then
> placed on the cold bar for freezing the oil embedding medium.
> 
> The oil block sections just as OCT or CryoGel does. This is a method used in
> several labs with the Tape-Transfer system.
> 
> Bernice
> schiller@instrumedics.com
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 16:57:07 -0500
> From: "Sarah Christo" <schristo@cvm.tamu.edu>
> Subject: Re: immuno sensitivity
> 
> Dear Judy,
>    I had the same problem in brain tissue trying to stain for serotonin.  I
> tried the BioGenex StrAviGen Super Sensitive Link & Label kit and finally had
> some reaction.  
>   
> 
> Sarah Christo, HT (ASCP)
> Texas A&M University
> College of Veterinary Medicine
> Dept. of Vet. Anatomy & Public Health
> College Station, TX  77868-4458
> schristo@cvm.tamu.edu
> 
> >>> Judy Trogadis <judy@playfair.utoronto.ca> 05/18 10:59 AM >>>
> Histonetters,
> 
> I am trying to detect a protein in brain tissue using immunocytochemistry.
> From northern and western blots we know that the RNA is made and is 
> translated into protein, now we want a more precise localization - it should
> be nuclear but we are not sure in which cell type(s) it is expressed.
> 
> I have used a biotinylated IgG followed by the ABC kit from Vector which is 
> a peroxidase reaction using DAB. No protein was detected although grossly,
> the tissue had more of a yellowish tint compared to negative controls. Also,
> we have a positive control which uses the same tissue preparation, i.e.
> fixation, cryosectioning, etc. One current theory is that the protein is      
> present in many cell types but at a low level of expression.
> 
> What would you say is the most sensitive immuno-detection method available?
> Would fluorescence be better?
> 
> Thanks in advance
> judy
> 
> 
> Judy Trogadis
> Eye Research Institute and
> University of Toronto
> Toronto Hospital, Western Div.
> 399 Bathurst  St.
> Toronto, Canada M5T 2S8
> 
> phone: 416-603-5088
> Fax:   416-603-5126
> email: judy@playfair.utoronto.ca 
> 
> 
> 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 16:57:43 -0500
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: NovaRed, Vector
> 
> I have a NovaRed from Vector, just starting to use it.  Zymed must have 
> a new substrate/chromogen also.  The Vector product is for HRP systems,
> 5 times more sensitive than AEC, equivalent to DAB, a brick red, and
> can be dehydrated through alcohols, clearants and permanent mounting media.
> What a joy! to be able to handle a red end product like DAB!
>   
> I found that with one antibody I was using, I will have to do 
> further dilutions on the primary.  And this will probably be the case with
> other antibodies as well.  The color developed FAST, maybe too much so.  
> 
> Gayle Callis
> 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 16:58:30 -0500
> From: Gwin Kerce <gkerce@arches.uga.edu>
> Subject: processing cost survey
> 
> 
> Help! Help! (I can't spell)
> I have done a cost analysis for my lab using a reference from Tech
> Sample ASCP ( Barbara Davies, pub.1996).I need to compare cost with
> other institutions for a grant proposal. All replies will be
> confidential.
> 
> Please answer the following questions:
>    Reagents                         Cost and quantity of
> reagents?              Quantity need to fill processor?
> 10% Formalin
> 
> 100% ETOH
> 
> 95% ETOH
> 
> Xylene
> 
> Paraffin
> 
> What is the average number of  blocks run through the processor between
> reagent changes?
> 
> 
> What is the cost of your processor service contract?
> 
> 
> How many blocks can you embed in 1K (1.8L) of paraffin?
> 
> 
> Thank you,
> Gwin Kerce
> UGA
> Dept. Avian Medicine PDRC
> Athens GA 30602
> ph# 706-542-7262
> fax# 706-542-5630
> 
> 
> 
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
> 
> 
> - ------------------ MIME Information follows ------------------
> 
> 
> - --------------A0DFECFDF073E5F5C9A7CB15
> Content-Type: text/plain; charset=us-ascii
> Content-Transfer-Encoding: 7bit
> 
> <<<<<< See above "Message Body" >>>>>>
> 
> - --------------A0DFECFDF073E5F5C9A7CB15
> Content-Type: text/html; charset=us-ascii
> Content-Transfer-Encoding: 7bit
> 
> <!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
> <HTML>
> Help! Help! (I can't spell)
> <BR>I have done a cost analysis for my lab using a reference from Tech
> Sample ASCP ( Barbara Davies, pub.1996).I need to compare cost with other
> institutions for a grant proposal. All replies will be confidential.
> <P>Please answer the following questions:
> <BR>  
> <U>Reagents </U>                       
> <U>Cost and quantity of
> reagents?</U>             <U>
> Quantity need to fill processor?</U>
> <BR>10% Formalin
> <P>100% ETOH
> <P>95% ETOH
> <P>Xylene
> <P>Paraffin
> <P>What is the average number of  blocks run through the processor
> between reagent changes?
> <BR> 
> <P>What is the cost of your processor service contract?
> <BR> 
> <P>How many blocks can you embed in 1K (1.8L) of paraffin?
> <BR> 
> <P>Thank you,
> <BR>Gwin Kerce
> <BR>UGA
> <BR>Dept. Avian Medicine PDRC
> <BR>Athens GA 30602
> <BR>ph# 706-542-7262
> <BR>fax# 706-542-5630</HTML>
> 
> - --------------A0DFECFDF073E5F5C9A7CB15--
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 16:58:57 -0500
> From: "Carol Bobrowitz" <Carol_Bobrowitz.PATHOLOGY@qmail.path.mcw.edu>
> Subject: Ag Retrieval, Waterbath
> 
>                       Subject:                              Time:  2:17 PM
>   OFFICE MEMO         Ag Retrieval, Waterbath               Date:  5/18/99
> Our lab is strictly an IHC lab and research lab.
> We use a waterbath for all Antigen Retrieval.  
> I purchased a large waterbath, measurements 15" long, 14" wide and 9" high,
> with a cover.
> We only use distilled water in it. 
> We have 2 metal Tissue Tek Cytology staining racks (room for 6 white Tissue
> Tek
> containers).  
> These racks are never removed from the water bath.  
> Slides are placed in the gray Tissue Tek Staining Racks (up to twenty slides).
> 
> Written on several different white staining containers the specific antigen
> retrieval and pH is listed.  (If a small number of slides need ag retrieval,
> Nalgene white coplin jars are used (no more than 4 slides per coplin jar).
> A thermometer is in the waterbath to maintain a 97#161#C to 100#161#C at all
> times.  
> Two problems we had and the solutions.
> 1)  Evaporation: A MUST, Daily, the end of the day, the water must be filled
> with distilled water over the metal staining racks.  
> 2)  Time waiting for the waterbath to reach 97#161#C to 100#161#C.  I
> purchased from
> two different scientific companies timers that you can set to your needs.
> The waterbath comes on at 4:00 am Monday thru Friday and is ready to use by
> 6:30 am and goes off at 2:00 pm.   Holiday weekends can also be set.
> Since using a the waterbath instead of a microwave our staining results have
> been excellent.  
> One MAJOR PROBLEM I have had:  Technicians rush to start the slides and do not
> pay attention to the waterbath temperature.  The slides were started below
> required temperature.  The results were not consistant and very poor quality. 
> TIP:  We have two MOPEC slide dryers.  Both dryers are on timers.  If slides
> are cut at the end of the day,  they are ready to start at 6:30 am.  We have
> at
> times put our floatation waterbaths on times.
> I hope this information helps.
> Carol Ann Bobrowitz
> Medical College of Wisconsin  
> 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 16:59:28 -0500
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: brushes
> 
> another source for brushes is  (if not mentioned before!)
> 
> Energy Beam Sciences
> 
> Gayle Callis :)
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 18:04:58 -0500
> From: "Jim Staruk" <jstaruk@masshistology.com>
> Subject: Re: waterbaths
> 
> Yes, a GE skillet from Wal-Mart.  Costs about $19.00 and keeps a perfect 42
> degree C temperature year after year!
> 
> Jim
> _________________________
> James E. Staruk, HT(ASCP)
> Mass Histology Service
> http://www.masshistology.com
> - -----Original Message-----
> From: Melody Ricci <melodyricci@usa.net>
> To: Histonet Mailing List <histonet@Pathology.swmed.edu>
> Date: Tuesday, May 18, 1999 5:50 PM
> Subject: waterbaths
> 
> 
> Thanks for all the protocols on waterbath antigen retrieval.
> 
> Speaking of waterbaths, I'm currently shopping for a new one.  Our Shandon
> died and they no longer carry the computer board for the digital temperature
> settings.  Does anyone recommend any other brand of waterbaths that display
> the digital temperature?
> 
> Thanks for the help!
> 
> M.Ricci
> 
> 
> ____________________________________________________________________
> Get free e-mail and a permanent address at http://www.amexmail.com/?A=1
> 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 21:16:07 -0500
> From: LMGephart@aol.com
> Subject: Re: Pain FROM the lab
> 
> I dunno...the first time one of my techs comes to work smelling of "au de 
> vinegar", I think I would send them home for a quick shower.  We work too 
> closely for that.
> 
> - -The sensitive nose
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 22:03:22 -0500
> From: "D. Hammer" <hammerd@u.washington.edu>
> Subject: Re: Pain FROM the lab
> 
> The Sensitive Nose:
> 
> I guess a good Italian lunch "ala garlic", good crusty bread dipped in
> Balsamic Vinegar, crushed garlic and olive oil, sprinkled with Romano
> cheese is out in your lab.  (never had a vampire strike me) :) 
> 
> Nose plugs are needed for the super sensitive.  I'm thinking of using 
> them against purfume/cologne at the theater or any other public
> gatherings...and they say smoking is a problem? :)  Why do people pour on
> the purfume/cologne? 
> 
> Methinks we make too much of stinks. 
> 
> Is anyone not sensitive to something nowadays or do we need an outlet for
> frustrations of society?
> 
> Don
> P.S. Why do buses still emit the fumes our cars do not because we have
> emission controls.  Just give me vinegar and garlic anyday and send ME
> home to cook. :)  If you have problems with smells, contact me for a
> product I became aware of recently...it will take out the smells of normal
> life in your homes and labs.  (but do it via donh7@earthlink.net, not this
> address)
>                                               
>                                              
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Don Hammer, Administrative Director            UNIVERSITY OF WASHINGTON 
> Hospital Pathology, Box 356100                     MEDICAL CENTER
> 1995 NE Pacific St.                                
> Seattle Washington, 98195                  ~Where Knowledge Comes To Life~ 
> (206) 598-6401 Fax: (206) 598-4928         
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> 
> 
> On Tue, 18 May 1999 LMGephart@aol.com wrote:
> 
> > I dunno...the first time one of my techs comes to work smelling of "au de 
> > vinegar", I think I would send them home for a quick shower.  We work too 
> > closely for that.
> > 
> > -The sensitive nose
> > 
> > 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 22:18:25 -0500
> From: Mary L North <northstar44@juno.com>
> Subject: Stain for atheromatous plaques
> 
> Is there a staining procedure available to detect the various components
> of an atheromatous plaque?  We will perform the Oil Red O on a frozen
> section to detect fat but are looking for the best technique, or panel of
> techniques,  to use on the paraffin sections.
> Mary North
> Loma Linda University Medical Center
> Loma Linda, CA
> 
> ___________________________________________________________________
> You don't need to buy Internet access to use free Internet e-mail.
> Get completely free e-mail from Juno at http://www.juno.com/getjuno.html
> or call Juno at (800) 654-JUNO [654-5866]
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 18 May 1999 22:19:15 -0500
> From: fetching@webtv.net (bonnie greer)
> Subject: Re: Pain FROM the lab
> 
> 
> Darn now I am hungry..........I think a vinegar/wine smell.beats the
> Xylene smell plus it will be more fun with wine WORK I mean.
> 
> 
> 
> 
> ******************* NOTE *******************
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> Received: from mailsorter-101-2.bryant.webtv.net (209.240.198.96) by
> 	postoffice-172.iap.bryant.webtv.net; Tue, 18 May 1999 20:09:00
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> Date: Tue, 18 May 1999 19:56:28 -0700 (PDT)
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> <Pine.A41.4.10.9905181932360.432354-100000@aagaard03.u.washington.edu>
> From: "D. Hammer" <hammerd@u.washington.edu>
> Subject: Re: Pain FROM the lab
> To: LMGephart@aol.com
> Cc: lburrell@pathbox.wustl.edu, HistoNet@Pathology.swmed.edu
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> The Sensitive Nose:
> 
> I guess a good Italian lunch "ala garlic", good crusty bread dipped in
> Balsamic Vinegar, crushed garlic and olive oil, sprinkled with Romano
> cheese is out in your lab.  (never had a vampire strike me) :) 
> 
> Nose plugs are needed for the super sensitive.  I'm thinking of using 
> them against purfume/cologne at the theater or any other public
> gatherings...and they say smoking is a problem? :)  Why do people pour on
> the purfume/cologne? 
> 
> Methinks we make too much of stinks. 
> 
> Is anyone not sensitive to something nowadays or do we need an outlet for
> frustrations of society?
> 
> Don
> P.S. Why do buses still emit the fumes our cars do not because we have
> emission controls.  Just give me vinegar and garlic anyday and send ME
> home to cook. :)  If you have problems with smells, contact me for a
> product I became aware of recently...it will take out the smells of normal
> life in your homes and labs.  (but do it via donh7@earthlink.net, not this
> address)
>                                               
>                                              
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Don Hammer, Administrative Director            UNIVERSITY OF WASHINGTON 
> Hospital Pathology, Box 356100                     MEDICAL CENTER
> 1995 NE Pacific St.                                
> Seattle Washington, 98195                  ~Where Knowledge Comes To Life~ 
> (206) 598-6401 Fax: (206) 598-4928         
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> 
> 
> On Tue, 18 May 1999 LMGephart@aol.com wrote:
> 
> > I dunno...the first time one of my techs comes to work smelling of "au de 
> > vinegar", I think I would send them home for a quick shower.  We work too 
> > closely for that.
> > 
> > -The sensitive nose
> > 
> > 
> 
> 
> 
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> Here are the messages received yesterday!
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