Re: Bone Marrow

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From:Teyluv@aol.com
To:histo20@hotmail.com, histonet@Pathology.swmed.edu
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Date:Tue, 25 May 1999 16:18:20 EDT
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Paula,

We treat our bone marrow core bxs in the following manner:
					1.  formalin 
fix...........................3 hours
					2.  Surgipath Decal 
II...............2.5 hours
					3.  rinse*(tap 
water)...............15 min.
      *Rinse is not necessary according to Surgipath label, but we do it by 
pathologist request.
					4.  process on routine overnight run.

	We are then able to get decent quality sections of cores at 2 
microns, although occasionally we have experienced a "blanking" effect in the 
nuclei of RBC precursor cells....(suggestions on that, anyone?)

	As for aspirates, we receive syringe clots and "unit" clots (which 
contain spicules).  They are fixed in 10% formalin, filtered into mesh 
baggies, cassetted and run on the routine processor.

	Hope I had some info that can help!  

			Liz Volm
			Methodist Hospital
			St. Louis Park, MN
			



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