Re: Bone Marrow

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From:"Bill Sinai (Anatomical Pathology)" <>
To:"Paula Wilder" <>,,
Date:Tue, 25 May 1999 09:29:20 +1000EST
Content-Type:text/plain; charset=US-ASCII

Date:          Sun, 23 May 1999 17:28:04 PDT
From:          "Paula Wilder" <>
Subject:       Bone Marrow

Bone Marrows,

We have the haematology registrar place the trephine immediately into 
FAA.  We then fix for a minimum of 4 hours and a maximum of 24hrs.

Decalcification is by 5% Nitric acid for two hours after fixation.

Process through an overnight schedule.

Cut sections at 2micron.

Stain H&E's and reticulin as standard stains.  Any immunoperoxidase 
are sectioned onto Superfrost Plus slides.

Hope this helps.
 Hello Histonetters!

Just a quick query - how is everyone handling their bone marrow biopsies and 
bone marrow aspirates?  Any responses would be greatly appreciated!  THANK 

Paula Wilder
St. Joseph Medical Center
Towson, MD. 21204

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Bill Sinai
Department Manager
Tissue Pathology
ICPMR Westmead Hospital 
Phone 61+2+9845 7774  Fax 61+2+9687 2330

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