Re: Bone Marrow

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From:"Bill Sinai (Anatomical Pathology)" <Bills@icpmr.wsahs.nsw.gov.au>
To:"Paula Wilder" <histo20@hotmail.com>, HistoNet@Pathology.swmed.edu, bills@icpmr.wsahs.nsw.gov.au
Reply-To:
Date:Tue, 25 May 1999 09:29:20 +1000EST
Content-Type:text/plain; charset=US-ASCII

Date:          Sun, 23 May 1999 17:28:04 PDT
From:          "Paula Wilder" <histo20@hotmail.com>
Subject:       Bone Marrow
To:            histonet@Pathology.swmed.edu

Bone Marrows,

We have the haematology registrar place the trephine immediately into 
FAA.  We then fix for a minimum of 4 hours and a maximum of 24hrs.

Decalcification is by 5% Nitric acid for two hours after fixation.

Process through an overnight schedule.

Cut sections at 2micron.

Stain H&E's and reticulin as standard stains.  Any immunoperoxidase 
are sectioned onto Superfrost Plus slides.

Hope this helps.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 Hello Histonetters!

Just a quick query - how is everyone handling their bone marrow biopsies and 
bone marrow aspirates?  Any responses would be greatly appreciated!  THANK 
YOU!

Paula Wilder
St. Joseph Medical Center
Towson, MD. 21204


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Bill Sinai
Department Manager
Tissue Pathology
ICPMR Westmead Hospital 
WESTMEAD NSW AUSTRALIA
Phone 61+2+9845 7774  Fax 61+2+9687 2330



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