protect RNA is a new product. Catalog number R7397 (30 ml of 500X solution )

----------
From:  Carpenter, Judith A.
Sent:  יום שלישי 18 מאי 1999 13:44
To:  'DORIT ZHARHARY'
Subject:  RE: water quality

Dear Dorit-
Would you have a catalog # for the "protect-RNA", all I can find in my
Sigma catalog is something called "RNase ZAP" for cleaning glassware
and plastics (cat#R2020).
Thanks very much-
Jude
Jude Carpenter,BS, HTL(ASCP)
Chief Technologist/Surgical Pathology/Histology/Autopsy
FAHC/MCHV Campus
111 Colchester Ave.
Burlington,   VT  05401
(802)656-5116
FAX : (802)656-3509
jude.carpenter@vtmednet.org


> -----Original Message-----
> From:	DORIT ZHARHARY [SMTP:d_zharhary@sigma.co.il]
> Sent:	Tuesday, May 18, 1999 3:17 AM
> To:	histonet@Pathology.swmed.edu; 'Heike Grabsch'
> Subject:	RE: water quality
> 
> It is better to use ultrapure water, however for in-situ hybridization (or
> any procedure where you do not need enzymatic activity) you do not have to
> treat the water with DEPC+ autoclave or autoclave any of your glassware,
> but instead add an RNase inhibitor to all solutions. This saves a lot of
> time. Such inhibitors, unlike those that are added where enzymatic
> activity is required, are relatively cheap, one example being a new
> product from Sigma called "protect-RNA".
> 
> Dorit
> 
> ----------
> From:  Heike Grabsch
> Sent:  יום שני 17 מאי 1999 04:53
> To:  histonet@Pathology.swmed.edu
> Subject:  water quality
> 
> A question for people who are doing studies with RNA like RT-PCR and in 
> situ hybridization for RNA:
> 
> what sort of water do you use?
> a) regular distilled water that is treated with DEPC and then autoclaved
> or 
> b) ultrapure water (f.ex. MilliQ)that is treated with DEPC and then 
> autoclaved
> 
> one of my collegues says that there is no need for ultrapure water, but I 
> would like to have some more opinions before I cut down my standards. (In 
> the lab where I was trained in molecular biology everything (also non-RNA 
> buffers etc) was prepared with ultrapure water.
> 
> thanks for some arguments
> 
> Dr. Heike Grabsch, Germany
>