I would recommend mouse on mouse kits that are specifically designed to
overcome background from endogenous mouse IgG. Zymed markets one called
HistoMouse, ScyTek also has one.

Brett


Brett M. Connolly, Ph.D.
Senior Research Biologist
Dept. of Human Genetics
Merck Research Laboratories
WP26A-3000
PO Box 4
West Point, PA 19486
tel. 215-652-2501
FAX 215-652-2075


> ----------
> From: 	Daniel Jimenez[SMTP:dfjimenez@ucdavis.edu]
> Sent: 	Wednesday, May 26, 1999 7:55 PM
> To: 	HistoNet
> Subject: 	mouse monoclonal in mouse tissues
> 
> 
> 
> 
> I am currently using a mouse primary antibodies in mouse tissues, but I
> have high background probably due to the inability of the
> anti-mouse-secondary antibody to distinguish between the primary antibody
> and the endogenous mouse Ig in the tissue. 
> Does anybody know how to eliminate the backgroung problem without changing
> the antibody? 
> 
> Thank you
> 
> Best Regards,
> 
> Daniel J
> 
> -------------------------------------------------------
> Daniel Jimenez
> University of California - Davis
> Dept of Pathology, Microbiology and Immunology
> Bldg Haring Hall, Room # 1126
> Davis, CA 95616
> tel (530) 7521183
> email dfjimenez@ucdavis.edu
> --------------------------------------------------------
> 
>