We use the Dako antibody, and have no trouble at all.  No enzyme digestion
or HIER.  If you need more information...let me know.

Sheila Tapper
Anatomic Pathology Team Leader
SMDC Health System
Duluth, MN
Stapper@smdc.org <mailto:Stapper@smdc.org> 


	-----Original Message-----
	From:	Liz Sabin [SMTP:esabin@srv4.med.ed.ac.uk]
	Sent:	Thursday, May 20, 1999 9:11 AM
	To:	Histonet@Pathology.swmed.edu
	Subject:	Mast Cells

	Hi all,
	Can anyone suggest an antibody to human Mast Cells which will work
on 
	paraffin sections?  The only one I've seen is to Mast Cell Tryptase,

	which both Dako and Novocastra sell.  Has anyone had any experience 
	with these antibodies?  Any hints/tips?
	Thanks,
	Best wishes,
	Liz Sabin.






	Liz Sabin
	Department of Pathology
	University of Edinburgh
	Teviot Place
	Edinburgh
	EH8 9AG

	Tel. 0131 650 2960
	Fax. 0131 650 6528