RE: Giemsa

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From:"Hewlett Bryan (CMH)" <HEWLETT@EXCHANGE1.CMH.ON.CA>
To:"'HistoNet@Pathology.swmed.edu'" <HistoNet@Pathology.swmed.edu>, "'andreah@imclone.com'" <andreah@imclone.com>
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Date:Mon, 17 May 1999 11:37:57 -0400
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Andrea,

To prepare Giemsa stock, dissolve 0.5g powder in 33ml glycerol by
standing at 50-60C for 2 hours, add 33ml methanol(acetone free) and
allow the soln. to stand 24 hours. To stain dried blood smears, they
should be fixed in methanol for 2-3 mins then transferred to diluted
Giemsa ( try 1:20-1:50 in pH6.8 phosphate buffer) for 10-20 min.

Bryan

>----------
>From: 	andreah@imclone.com[SMTP:andreah@imclone.com]
>Sent: 	May 17, 1999 9:19 AM
>To: 	HistoNet@Pathology.swmed.edu
>Subject: 	Giemsa
>
>I was wondering if anyone has a protocol/recipe for staining blood smears
>with 
>Giemsa made up from powder.  I had ordered the kit from Shandon Lipshaw which
>is on backorder now for quite a while.  I have the powder but odn't know the 
>protocol. 
>
>Thanks in advance.
>Andrea
>andreah@imclone.com
>
>
>



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