RE: Giemsa

<< Previous Message | Next Message >>
From:"Hewlett Bryan (CMH)" <HEWLETT@EXCHANGE1.CMH.ON.CA>
To:"''" <>, "''" <>
Date:Mon, 17 May 1999 11:37:57 -0400
Content-Type:text/plain; charset="us-ascii"


To prepare Giemsa stock, dissolve 0.5g powder in 33ml glycerol by
standing at 50-60C for 2 hours, add 33ml methanol(acetone free) and
allow the soln. to stand 24 hours. To stain dried blood smears, they
should be fixed in methanol for 2-3 mins then transferred to diluted
Giemsa ( try 1:20-1:50 in pH6.8 phosphate buffer) for 10-20 min.


>Sent: 	May 17, 1999 9:19 AM
>Subject: 	Giemsa
>I was wondering if anyone has a protocol/recipe for staining blood smears
>Giemsa made up from powder.  I had ordered the kit from Shandon Lipshaw which
>is on backorder now for quite a while.  I have the powder but odn't know the 
>Thanks in advance.

<< Previous Message | Next Message >>