Hi Joe,

We use a variety of controls for CD34 depending on the application.  These
include uterus, tonsil, skin, positive tumors, i e ALL, early precursor
B-cell neoplasms, and positive tumors in bone marrow biopsies.

Linda Sebree, HT
University of Wisconsin Hospital & Clinics
Department of Laboratory Medicine
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI  53792-2472

Phone:  (608)265-6596
FAX:  (608)263-1568

> -----Original Message-----
> From:	Nocito, Joseph [SMTP:joseph_nocito@srhc.iwhs.org]
> Sent:	Monday, May 24, 1999 3:11 PM
> To:	'Histonet'
> Subject:	Control for CD-34
> 
> Hello everyone
> my pathologist and I are in a discussion on what to use as a control for
> CD-34(HPLA).  I say tonsil works well to identify the endothelial cells.
> He
> wants me to use spleen.  I've tried several spleen cases, but I still like
> the tonsil better.  The staining in the tonsil seems stronger to me than
> it
> does in the spleen.  Am I off base with this?  Time for a reality check I
> guess.
> 
> Joe Nocito, B.S., HT(ASCP)QIHC
> Histology Supervisor
> Christus Santa Rosa Hospitals
> San Antonio, Texas 
>  
> 
> Joe Nocito, B.S., HT(ASCP)QIHC
> Histology Supervisor
> Christus Santa Rosa Hospitals
> San Antonio, Texas 
>