RE: Ag Retrieval
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From: | "Johnson, Jennifer(Hist)" <jjohnson3@genzyme.com> |
To: | "'histonet'" <histonet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Tue, 18 May 1999 14:06:47 -0400 |
Content-Type: | |
Hi Melody,
I perform my antigen retrival in a plastic jar in a 90-99?C waterbath. I
use the solution from Vector called "Antigen Unmasking Solution" diluted
1:100 in water. I bring the solution to temperature, drop the slides in for
5-15 minutes (depending on the antibody), then remove the jar of slides from
the waterbath and let that sit out on the bench for 15 minutes to cool down.
Then I move on to the block or enzyme digestion or whatever step is next
required. It works like a charm on several of our collagen antibodies in
tough tissues like bone and cartilage.
Please contact me offline if you want any more info!
Jennifer Johnson
jjohnson3gGenzyme.com
> ----------
> From: Melody Ricci
> Sent: Monday, May 17, 1999 3:03 PM
> To: Histonet Mailing List
> Subject: Ag Retrieval
>
> I have always done antigen retrieval methods using the microwave, and I
> have
> read about those who use the veggie steamers. Has anyone out there done
> antigen retrieval in a waterbath? Would they be willing to share a
> protocol?
>
> Thanks!
>
> M.Ricci
>
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