RE: Ag Retrieval

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From:"Nocito, Joseph" <>
To:"'Melody Ricci'" <>, Histonet Mailing List <>
Date:Mon, 17 May 1999 15:07:10 -0500

I've used a large serologicial waterbath to perform epitope retrieval.  As
long as the waterbath can reach up to 95 degrees C, it should work.  I've
been using the steamer for over a year.  I have found that steaming is
easier on the tissue than microwaving.  Especially breast cases, bone
marrows and other difficult to work with tissue.  At the Texas Society for
Histotechnology annual meeting, Biocare Medical was demonstrating
a"Decloaking Chamber".  It is an electric pressure cooker.  I haven't tried
it yet, but I understand that it is a lot faster than steaming.  Good luck

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas 

> -----Original Message-----
> From:	Melody Ricci []
> Sent:	Monday, May 17, 1999 2:03 PM
> To:	Histonet Mailing List
> Subject:	Ag Retrieval
> I have always done antigen retrieval methods using the microwave, and I
> have
> read about those who use the veggie steamers.  Has anyone out there done
> antigen retrieval in a waterbath?  Would they be willing to share a
> protocol?
> Thanks!
> M.Ricci
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