Does anyone out there have any experience with different embedding media
for immunohistochemistry at the LM and TEM level?

At present I use EPON for TEM and LM (sometimes Historesin for LM) and
frozen sections for enzyme- and immunohistochemistry. One problem that
arises with the latter technique is that material in the lumen of gut
sections is washed away during the labelling procedure. I therefore want to
try an alternative embedding medium, that infiltrates the tissue and keeps
everything in place, and is suitable for immunohistochemistry AND
examinations at the TEM level. I have heard that LR White is an
alternative, but I do not have any experience with it. What are the
advantages (or disadvantages) of this medium compared to others, such as
Unicryl? I need information about polymerisation temperature, special
equipment needed, special procedures before labelling, etc.

Thank you in advance for your help!




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Tora Bardal					tlf: + (47)73 59 09 38
Department of Zoology, 			fax: + (47)73 59 63 11
Norwegian University of Science and Technology (NTNU)
Brattoera Research Center
N-7491 Trondheim
Norway