IHC on frozen sections

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From:"Coskran, Timothy M" <timothy_m_coskran@groton.pfizer.com>
To:"'Histonet@pathology.swmed.edu'" <Histonet@Pathology.swmed.edu>
Date:Tue, 18 May 1999 14:37:55 -0400
Content-Type:text/plain; charset="iso-8859-1"


Lately, I've had a problem with immuno on frozen sections.  There is a great
deal of background staining showing up on my negative control. I'm not sure
why this is happening since we use the same secondary antibodies and labels
for paraffin and don't see this problem.  The problem is occurring with both
DAB and Histomark red.  Any ideas?

Tim Coskran

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