IHC Substrates
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From: | "vector@vectorlabs.com" <vector@vectorlabs.com> |
To: | "Histonet" <Histonet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Wed, 19 May 99 10:06:46 -0700 |
Content-Type: | text/plain; charset="US-ASCII" |
We are responding to the inquiry posted earlier from Barb Terrett,
concerning the difference between the Vector NovaRED (Cat. No. SK-4800)
and Vector Red (Cat. No. SK-5100) substrates. We would like to confirm
that the reply from Gayle Callis on this matter is certainly correct.
Just for a further point of clarification, these substrate kits are
offered as separate units, not as part of a complete "staining" kit, and
they can be applied to any peroxidase or alkaline phosphatase detection
system, respectively.
The Vector NovaRED was developed to provide a red peroxidase substrate
which could be dehydrated through ethanols, cleared and permanently
mounted. The reaction kinetics of Vector NovaRED are similar to DAB, and
it produces a red reaction product which is 5x greater in sensitivity
than AEC.
As Gayle indicated, the Vector Red is a red alkaline phosphatase
substrate which also can be dehydrated, cleared and permanently mounted.
The Vector Red is unusual in that it can be viewed as a precipitate under
standard light microscopy, and it is also fluorescent.
We hope this clears up any confusion.
Sincerely,
Technical Services
Vector Laboratories
30 Ingold Road
Burlingame, CA 94010
Tel: (650) 697-3600
Fax: (650) 697-0339
E-mail: vector@vectorlabs.com
Website: http://www.vectorlabs.com
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