Fixation of frozen sections

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From:"Coskran, Timothy M" <>
To:"''" <>
Date:Thu, 27 May 1999 15:33:54 -0400
Content-Type:text/plain; charset="iso-8859-1"

Does anyone have any ideas on whether it is better to cut frozens, fix and
store the sections or to cut frozens, store, and then fix when your ready to
carry out a stain?  We currently cut frozen sections, dry briefly and then
store the slides at -80C until we are ready to do an immuno stain.  Then we
bring the slides to room temp, fix and proceed with the stain.  I've seem
some literature that suggests fixing the slides immediately after they have
been sectioned and then storing in PBS until ready to use.

If anyone has a basic protocol for frozen sections, would they be willing to


Tim Coskran

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