DAB film

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From:Brandon Schanbacher <schanbacher.2@osu.edu>
To:"'histonet@pathology.swmed.edu'" <histonet@Pathology.swmed.edu>
Date:Tue, 18 May 1999 13:47:04 -0400
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Hello all,

We have had some problems with DAB solution leaving a light brown haze
across the entire slide during immunostaining.  If anyone has any
suggestions on how to remedy this situation, I'd appreciate any input.

The haze is most apparent when we use Upstate Biotechnologies' anti
3-nitrotyrosine antibody.  As far as our staining procedure, we use the
Microprobe capillary gap system, which made us think it was a problem
rinsing one of the antibody steps off of the slide.  We have increased
the buffer rinses to no avail.  We don't get this film using other
antibodies run at the same time, in the same DAB solution, so I doubt
it's a problem with the DAB solution.  Has anyone else run into a
similar situation?


Brandon Schanbacher
Division of Pharmacology
Ohio State University

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