Hello All,

I've just been handed a journal article from 1977 with a 
histochemical method for carbonic anhydrase (hydro-lyase).
The method uses 20-25 um frozen sections and a barrage of nasty 
chemicals with many manipulations of free floating tissues. Lucky me 
eh? 

Anyway, I'm hoping, no, praying that there has been a better way developed 
in the years since, to demonstrate carbonic anhydrase. IHC maybe?? 
Anyone out there with any experience with this one?? 

                                                                  Cheers!  Greg 
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Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 Unviversity Ave.
Charlottetown, P.E.I.
C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851