Dear Histonetters:

I will be doing some p53 immunohistochemistry in the near future. The
protocol I have require fixation in 3.7% formalin, then paraffin sectioning.
My questions are:
1. How stable is p53 after formalin fixation in other words how long can I
wait until I do the staining?

2. I have only equipment to do frozen sections, is there any protocol to
perform frozen sections for p53? What is the fixative?. I may have the
possibility to send the tissue to be sectioned to an outside lab, but to do
that I need to know the stability of p53.

I will appreciate your always insightful comments.

Nelson


Nelson Aguila, D.V.M
Senior Research Scientist
RPR-Gencell
Oncology Research
Phone (510) 266-5030
Nelson.Aguila@rp-rorer.com