Tissue culture background

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From:"Barry, Lilith" <Lilith.Barry@nrc.ca>
To:Histonet <histonet@Pathology.swmed.edu>, microscopy <microscopy@Sparc5.Microscopy.Com>
Date:Tue, 4 May 1999 14:48:00 -0400

A colleague of mine works on primary cortical cultures. When she does
immunocytochemistry on them she usually uses indirect  (immunofluorescence)
method, which works well.   When she uses  fluorescence labeled
avidin-biotin method and she gets extremely high background. I have looked
at the controls. The non specific binding is as bright as the specific one.
I have never worked with cultures. Could there be endogenous biotin in them?
Or would you have any other suggestions on what could be the cause and what
can one do to prevent this?
Thanking you in advance for your input,
Lilith Ohannessian-Barry
National Research Council
Institute of Biological Sciences
e-mail; lilith.barry@nrc.ca

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