| From: | "Barry, Lilith" <Lilith.Barry@nrc.ca> |
| To: | Histonet <histonet@Pathology.swmed.edu>, microscopy <microscopy@Sparc5.Microscopy.Com> |
| Reply-To: | |
| Date: | Thu, 6 May 1999 09:54:00 -0400 |
| Content-Type: | text/plain |
Thank you for responding to my question on tissue culture background. I don't think the background is because the fixation or the immuno method because I routinely use the same method on brain sections and do not get the same background. It isn't the coverslip autofluorescing either because one can see beautiful labeled structures in the cells except on the controls. So it has to be something specific about cortical cultures and the strept or neutravidin-biotin method. Do you have any other thoughts? Lilith ------------------------------------------------------------------------ Lilith Ohannessian-Barry National Research Council Institute of Biological Sciences CANADA Tel;613-993-6460 Fax;613-941-4475 e-mail; lilith.barry@nrc.ca