Tissue culture background- Thank you

<< Previous Message | Next Message >>
From:"Barry, Lilith" <Lilith.Barry@nrc.ca>
To:Histonet <histonet@Pathology.swmed.edu>, microscopy <microscopy@Sparc5.Microscopy.Com>
Date:Thu, 6 May 1999 09:54:00 -0400

Thank you  for responding to my question on tissue culture background. I
don't think the background is because the fixation or the  immuno method
because I routinely use the same method on brain sections and do not get the
same background. It isn't the coverslip autofluorescing either because one
can see beautiful labeled structures in the cells except on the controls. So
it has to be something specific  about cortical cultures and the strept or
neutravidin-biotin method. Do you have any other thoughts?
Lilith Ohannessian-Barry
National Research Council
Institute of Biological Sciences
e-mail; lilith.barry@nrc.ca

<< Previous Message | Next Message >>