Safranin O stain, Bone fixation

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Date:Wed, 5 May 1999 08:40:03 -0700
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Do you know of any publications that explain the theory and principles of
the Safranin O/Fast Green stain?  I follow the procedure from the AFIP
manual but have been unable to find any additional information.

I am also interested in feedback on fixation of bone specimens.  I work
with rabbit & sheep knees and am currently fixing with 10% formalin and
Surgipath Decalcifier I.  It seems to be working fine but I have been asked
to try different fixation treatments.  Does any one fix bone with something
other that 10% formalin?

Thanks for your help.

Rose Bellantoni
   Senior Research Associate
   Integra Life Sciences, CRC
   11045 Roselle St.
   San Diego CA 92121-2199

  tel:  619-622-2740
  fax: 619-535-8269

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