Histonetters,

>From my old days of doing titrations in analytical chemistry lab, I learned that a 
"drop" is approximately 50 microliters.  This is a real ballpark figure; depends 
on delivery tip, ambient breezes, method of delivery, etc.  So we always take 
those darn lids off the Vector bottles, and use 10 microliters of A and B per mL.  
Works great.  Saves reagents.  We can stain a 10-20 embryos using 100 microliters 
of ABC reagent.

Karen -- University of Oregon



Date:          Tue, 11 May 1999 15:47:02 -0800
From:          Nora Fox <nefox@leland.Stanford.EDU>
Subject:       Re: squeeze bottles/painful finger joints
To:            histonet@Pathology.swmed.edu

For those prefering to pipette rather than squeeze, I suppose that you
could use a good balance & a known volume from a well calibrated pipette to
determine volume per drop.

--just an idea.

Thanks for all the great discussions & advice.

Nora