Re: plastic-embedded tissues

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From:Katie B <>
To:Bret Cook <>,
Date:Tue, 11 May 1999 08:22:27 -0700 (PDT)
Content-Type:text/plain; charset=us-ascii

I have been doing IHC in GMA with lots of success. 
Our lab uses Polysciences Immuno-bed kit which is a
GMA based plastic but is specially formulated to be
used in light microscopy immunohistochem procedures. 
I then adapt my paraffin IHC protocol to longer
(usually double) incubation times at 37 degrees
instead of room temp.  However, if the paraffin
protocol calls for a primary antibody incubation of
overnight at 4 degrees I would keep it at that.

--- Bret Cook <> wrote:
> Hello everyone,
> I seem to remember some discussion on
> plastic-embedded tissues in the past.
> Does anyone have a procedure for IHC on glycol
> methacrylate-embedded tissue.
> I am wondering what kind of pre-treatment is
> required or what works best.
> Any tips would be great.
> Thanks a bunch,
> Bret Cook
> DAKO Corporation

Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

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