Re: plastic-embedded tissues
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From: | Katie B <bresee98@yahoo.com> |
To: | Bret Cook <bret.cook@dakousa.com>, histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Tue, 11 May 1999 08:22:27 -0700 (PDT) |
Content-Type: | text/plain; charset=us-ascii |
I have been doing IHC in GMA with lots of success.
Our lab uses Polysciences Immuno-bed kit which is a
GMA based plastic but is specially formulated to be
used in light microscopy immunohistochem procedures.
I then adapt my paraffin IHC protocol to longer
(usually double) incubation times at 37 degrees
instead of room temp. However, if the paraffin
protocol calls for a primary antibody incubation of
overnight at 4 degrees I would keep it at that.
--- Bret Cook <bret.cook@dakousa.com> wrote:
> Hello everyone,
>
> I seem to remember some discussion on
> plastic-embedded tissues in the past.
>
> Does anyone have a procedure for IHC on glycol
> methacrylate-embedded tissue.
> I am wondering what kind of pre-treatment is
> required or what works best.
> Any tips would be great.
>
> Thanks a bunch,
>
> Bret Cook
> DAKO Corporation
===
Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University
*new* e-mail: bresee98@yahoo.com
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