Re: alcian stain for GMA

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From:Katie B <bresee98@yahoo.com>
To:Patsy.Ruegg@UCHSC.edu, lori.smith@sous.com, Histonet Server <histonet@Pathology.swmed.edu>
Reply-To:
Date:Tue, 11 May 1999 08:14:15 -0700 (PDT)
Content-Type:text/plain; charset=us-ascii

I have done Alcian Blue/Periodic Acid Schiff's stain
on GMA sections.  The trick is a 10 minute
pre-treatment of the slides with a Sodium Ethoxide
solution to etch away the plastic.  Rinse well in
running water before staining.

To make a sodium ethoxide solution, saturate 100%
ethanol with excess amounts of sodium hydroxide. 
Store at 4 degrees overnight to equilibrate.  The
sodium hydroxide should not all go into solution.  Be
very careful using this stuff and use it in a hood. 
The solution can be reused as long as it is stored at
-20 degrees and in a light tight container.  Discard
if color changes to brown.

My protocol is for staining is the same as for
paraffin after the sodium ethoxide step.

Good Luck!


===
Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

*new* e-mail: bresee98@yahoo.com

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