Re: alcian stain for GMA

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From:Katie B <>,, Histonet Server <>
Date:Tue, 11 May 1999 08:14:15 -0700 (PDT)
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I have done Alcian Blue/Periodic Acid Schiff's stain
on GMA sections.  The trick is a 10 minute
pre-treatment of the slides with a Sodium Ethoxide
solution to etch away the plastic.  Rinse well in
running water before staining.

To make a sodium ethoxide solution, saturate 100%
ethanol with excess amounts of sodium hydroxide. 
Store at 4 degrees overnight to equilibrate.  The
sodium hydroxide should not all go into solution.  Be
very careful using this stuff and use it in a hood. 
The solution can be reused as long as it is stored at
-20 degrees and in a light tight container.  Discard
if color changes to brown.

My protocol is for staining is the same as for
paraffin after the sodium ethoxide step.

Good Luck!

Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

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