Re: Tissue culture background- Thank you

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From:Katri Tuomala <>
To:"Barry, Lilith" <>
Date:Sun, 09 May 1999 21:00:56 -0400
Content-Type:text/plain; charset=us-ascii

Barry, Lilith wrote:
> Thank you  for responding to my question on tissue culture background. I
> don't think the background is because the fixation or the  immuno method
> because I routinely use the same method on brain sections and do not get the
> same background. It isn't the coverslip autofluorescing either because one
> can see beautiful labeled structures in the cells except on the controls. So
> it has to be something specific  about cortical cultures and the strept or
> neutravidin-biotin method. Do you have any other thoughts?
> Lilith
>  ------------------------------------------------------------------------
> Lilith Ohannessian-Barry
> National Research Council
> Institute of Biological Sciences
> Tel;613-993-6460
> Fax;613-941-4475
> e-mail;

Hi Lilith,
Have you ruled out the endogenous biotin? Either try a biotin block
prior to your staining or try another detection method, which does not
utilize biotin.

Katri Tuomala
Anatomic Pathgology
St.Joseph's Hospital
Hamilton, Ontario, Canada

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