Re: GMA/IHC/PMMA

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From:rschoonh@sph.unc.edu
To:uvsgc@msu.oscs.montana.edu (Gayle Callis), histonet@Pathology.swmed.edu
Reply-To:
Date:Tue, 11 May 1999 11:07:54 -0400 (Eastern Daylight Time)
Content-Type:TEXT/PLAIN; CHARSET=US-ASCII

I would like to echo Gails comments on GMA.  Unless you enjoy beating your head
against a brick wall  I wouldn't even try (yes I have tried, and yes, I hve the
bruises to prove it).  There are a FEW that will work but if you know in advance
the you need to do IHC Stick with PMMA or paraffin.

By the way Gail I did find something that would remove GMA.....unfortunatly it
also ate the glass slides.......;-)

best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone 
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123 

**Suppose you were an idiot... And suppose you were a member of Congress ...
But I repeat myself.-Mark Twain**

-- Begin original message --
> 
> GMA (glycol methacrylate) is a poor embedding media for IHC protocols
> in general, since you CANNOT remove the plastic which prevents 
> immunoglobulins from reaching antigenic sites.  Some people have success.
> 
> You need to refer to Neil Hand's article, in Journal of Histotechnology
> on "Using Superheating using pressure cooking: its use and application
> in unmasking antigens embedded in  methylmethacrylate" 21:231-236, Sept 1998
> 
> He microtomes PMMA (polymethyl methacrylate) embedded tissues, 
> has a huge panel of antibodies
> (well over a hundred) which he successfully stains.  He removes the 
> PMMA before staining, with wonderful results.  He has further references in
> the article and can be reached via email, he has tried GMA, plus many other
> plastics for IHC. mpznhand@unix.ccc.nottingham.ac.uk.  Give him a jingle  
> 
> Gayle Callis
> 
> 

-- End original message --




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