Re: GMA/IHC/PMMA
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From: | rschoonh@sph.unc.edu |
To: | uvsgc@msu.oscs.montana.edu (Gayle Callis), histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Tue, 11 May 1999 11:07:54 -0400 (Eastern Daylight Time) |
Content-Type: | TEXT/PLAIN; CHARSET=US-ASCII |
I would like to echo Gails comments on GMA. Unless you enjoy beating your head
against a brick wall I wouldn't even try (yes I have tried, and yes, I hve the
bruises to prove it). There are a FEW that will work but if you know in advance
the you need to do IHC Stick with PMMA or paraffin.
By the way Gail I did find something that would remove GMA.....unfortunatly it
also ate the glass slides.......;-)
best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
Lab 919-966-6140
Fax 919-966-6123
**Suppose you were an idiot... And suppose you were a member of Congress ...
But I repeat myself.-Mark Twain**
-- Begin original message --
>
> GMA (glycol methacrylate) is a poor embedding media for IHC protocols
> in general, since you CANNOT remove the plastic which prevents
> immunoglobulins from reaching antigenic sites. Some people have success.
>
> You need to refer to Neil Hand's article, in Journal of Histotechnology
> on "Using Superheating using pressure cooking: its use and application
> in unmasking antigens embedded in methylmethacrylate" 21:231-236, Sept 1998
>
> He microtomes PMMA (polymethyl methacrylate) embedded tissues,
> has a huge panel of antibodies
> (well over a hundred) which he successfully stains. He removes the
> PMMA before staining, with wonderful results. He has further references in
> the article and can be reached via email, he has tried GMA, plus many other
> plastics for IHC. mpznhand@unix.ccc.nottingham.ac.uk. Give him a jingle
>
> Gayle Callis
>
>
-- End original message --
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