RE: alcian stain for GMA

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From:"Kellar, Eric" <kellarec@MSX.UPMC.EDU>, "''" <>
Date:Tue, 11 May 1999 11:15:35 -0400

Alcian blue solution has a difficult time penetrating glycomethacrylate
resins because of its high particle weight  > 1000 daltons.

Give this method a try :

Technique: Methacrylate sections at  2-4 um.

Alcian Blue Solution:

Alcian Blue 8GX		0.5 g
Glacial acetic acid		3.0 ml
DH2O					100 ml
PH to 2.5

Nuclear fast red:

Nuclear fast red			 0.1 g
5% aluminum sulfate	100 ml
Dissolve with heat.. Cool and filter. Add grain of thymol as preservative.

1.	Place slide in plastic staining jar with 50 ml of Alcian blue
solution and irradiate at 450 W (80%) for 60 seconds. Allow the slide to
remain in the hot working solution for a few seconds until staining
intensity is achieved.
2.	Rinse well in distilled water.
3.	Drop nuclear fast red solution onto section and irradiate at 450W
(80%) for 10 seconds.
4.	Rinse in distilled water.
5.	Dehydrate in 95%,100%, clear and mount.

Eric C. Kellar
University of Pittsburgh Medical Center

	From: []
	Sent:  Monday, May 10, 1999 7:51 PM
	Subject:  alcian stain for GMA

	the lab is trying to do an Alcian blue on 4um glycol sections. 
	       Do you have a procedure for this.  None of the paraffin
	       working.  I can't seem to post this to the histonet, it
	       the message.  So I wondered if you had anything. 
	       Thanks...Lori  []  

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