RE: Spirochete staining kits

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From:"Kellar, Eric" <kellarec@MSX.UPMC.EDU>
To:HistoNet@Pathology.swmed.edu, "'VIKOHUT@aol.com'" <VIKOHUT@aol.com>
Reply-To:
Date:Fri, 7 May 1999 11:51:39 -0400
Content-Type:text/plain; charset="iso-8859-1"

Here are 5 commercially available staining kits that I have tried - all are
modified Steiner-Steiner techniques for (Spirochete, Legionella, Cat
Scratch, Helicobacter and Donovan Bodies). The staining results from all the
kits are quite comparable microscopically to one another, the biggest
difference is in price. We perform so many, that  it is more cost effective
for us to prepare our own Steiner solutions and stain using the microwave
technique.  We also prefer to substitute Zinc formalin for the Uranyl
nitrate that is sold as a sensitizer in these kits.

You may want to call for current pricing.

Steiner Modified IMEB stain kit - #K7374 120 ml
IMEB, Inc
San Marcos, CA
1-800-543-8496

Steiner (modified) technique for Spirochetes - #k069
PolyScientific
1-800-645-5825

Silver Stain, Modified Steiner-Steiner - #HT101-A
Sigma/Aldrich
1-800-325-3010

Steiner Stain Kit - #KTSTE
American Master Tech
Scientific, Inc.
PO Box 2539
Lodi, CA 95241-2539
1-800-860-4073

Silver (Modified Steiner-Steiner) Microwave Staining Kit - #MSK-101
Energy Beam Sciences, Inc.
11 Bowles Road  P.O. Box 468  Agawam, Massachusetts 01001
Phone: 800-992-9037 or 413-786-9322  Fax: 413-789-2786       


Eric C. Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center   







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