RE: Special Stains

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From:"Gary W. Gill" <>
To:"Andrea Kaj" <>, <>
Date:Tue, 11 May 1999 22:27:26 -0500
Content-Type:text/plain; charset="iso-8859-1"


	You are justified in being "a bit concerned where they got the protocols
from?"  As the "father" of Gill's hematoxylins and Gill's modified
Papanicolaou OG and EA stains, I can attest to your concern from first-hand
knowledge about the wide variability in such products among vendors and the
resulting confusion and variable results among Pap stain users.  On the
other hand, in defense of stain vendors, they often do not have in-house
stain expertise and are not in a position to evaluate the competency of
stain consultants.

Gary W. Gill

> -----Original Message-----
> From: Andrea Kaj []
> Sent: April 29, 1999 4:39 AM
> To:
> Subject: Special Stains
> Hi Netters
> Does anybody have a link to a page about special stains? I have
> been following the discussion about histotechnology/IHC being art
> or science, and it seems like we are forgetting the importance of
> standardising the 'old-fashioned' special stains. There is a lot
> of standardisation going on in IHC, but how about special stains?
> Any groups around?
> Some of the vendors are now offering sp. stain modules or
> reagents, but I am a bit concerned where they got the protocols
> from? And what happens when doing the stains at 60 degrees
> instead of room temp.? And how about disposal of various kinds of
> chemicals?
> Andrea
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