I fix bone with Shandon's Zink formol, it is great for IHC.  I decal with
formic acid (5%) which is also good for IHC, so I guess what you use to fix
and decal with depends on what you want to do.
Patsy

		-----Original Message-----
		From:	Rose_Bellantoni@integra-ls.com
[mailto:Rose_Bellantoni@integra-ls.com]
		Sent:	Wednesday, May 05, 1999 9:40 AM
		To:	histonet@Pathology.swmed.edu
		Subject:	Safranin O stain, Bone fixation

		Histonetters,

		Do you know of any publications that explain the theory and
principles of
		the Safranin O/Fast Green stain?  I follow the procedure
from the AFIP
		manual but have been unable to find any additional
information.

		I am also interested in feedback on fixation of bone
specimens.  I work
		with rabbit & sheep knees and am currently fixing with 10%
formalin and
		Surgipath Decalcifier I.  It seems to be working fine but I
have been asked
		to try different fixation treatments.  Does any one fix bone
with something
		other that 10% formalin?

		Thanks for your help.

		Rose Bellantoni
		   Senior Research Associate
		   Integra Life Sciences, CRC
		   11045 Roselle St.
		   San Diego CA 92121-2199

		  tel:  619-622-2740
		  fax: 619-535-8269
		  rose_bellantoni@Integra-LS.com