RE: Fixation for breast tissue

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From:"Bartlett, Jeanine" <jqb7@cdc.gov>
To:"'Terrett, Barb'" <Barb_Terrett@pmh.toronto.on.ca>, "'histonet@pathology.swmed.edu'" <histonet@Pathology.swmed.edu>
Reply-To:
Date:Thu, 6 May 1999 06:34:08 -0400
Content-Type:text/plain; charset="iso-8859-1"

Try Pen-Fix...it works very well.
Jeanine H. Bartlett, HT(ASCP)
Centers for Disease Control
NCID, DVRD, IDPA
1600 Clifton Rd., NE  MS/G-32
Atlanta, GA  30333


-----Original Message-----
From: Terrett, Barb [mailto:Barb_Terrett@pmh.toronto.on.ca]
Sent: Wednesday, May 05, 1999 3:00 PM
To: 'histonet@pathology.swmed.edu'
Subject: Fixation for breast tissue


I was wondering if there is anyone who uses a fixative for breast tissue
that is comprised of formalin, alcohol and/or acetic acid.  We are looking
for something that fixes fatty tissue better than buffered formalin.   If
you have such a fixative, could you share the recipe with us.   Also, does
this fixative have any negative effects on immuno staining, in particular
estrogen and progesterone receptors.

Thank you.   Sandra via Barb!




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