RE: Controlling fixation time
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From: | Patsy.Ruegg@UCHSC.edu |
To: | andreakaj@hotmail.com |
Reply-To: | |
Date: | Wed, 5 May 1999 12:19:13 -0600 |
Content-Type: | text/plain; charset="iso-8859-1" |
Andrea,
I have always thought that washing the sample in running di water would
rinse out the excess fixative and stop the reaction. I always do this
before starting decal, for instance.
Patsy
-----Original Message-----
From: Andrea Kaj [mailto:andreakaj@hotmail.com]
Sent: Wednesday, May 05, 1999 3:20 AM
To: HistoNet@Pathology.swmed.edu
Subject: Controlling fixation time
Science or art?
We all know that a perfect fixation is rarely accomplished
in the
routine....It is almost impossible to control the fixation
time from the
sample leaves the patient to landing on the pathologist's
table.
I am about to make some controlled fixations tests on fresh
tonsils (right
from the surgeons table), and I try to figure out how to
actually stop the
(formalin) fixation without damaging the tissue.
I suppose I could transfer the tissue into a mixture of
sucrose and
gummi-arabicum, but I would really appreciate any knowledge
from Histonet on
this subject.
Kind regards Andrea
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