Joseph,

We have tried and are now using Biocare's dual clone CD15 on our Ventana
immunostainers with very nice results.

Linda Sebree, HT
University of Wisconsin Hospital & Clinics
Department of Laboratory Medicine
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI  53792-2472

Phone:  (608)265-6596
FAX:  (608)263-1568

> -----Original Message-----
> From:	Nocito, Joseph [SMTP:joseph_nocito@srhc.iwhs.org]
> Sent:	Monday, May 10, 1999 4:12 PM
> To:	'Edward Henry'; histonet@Pathology.swmed.edu
> Subject:	RE: CD15 antibody
> 
> Edward,
> I received some literature from Biocare Medical on CD-15.  They have a
> monoclonal cocktail for CD-15 (clones DT07 + BC97).  You might give that a
> try.  Their number is 1-800-799-9499. I haven't tried it yet, but my
> understanding is that it works well in formalin-fixed tissue.  I'm using
> the
> B-D CD15 on my Dako autostainer at a titer of 1:100 with a 30 minute
> incubation.  Most of the tissue we have is B5-fixed. Hope this helps
> 
> Joe Nocito, B.S., HT(ASCP)QIHC
> Histology Supervisor
> Christus Santa Rosa Hospitals
> San Antonio, Texas 
> 
> 
> > -----Original Message-----
> > From:	Edward Henry [SMTP:HenryE@holycrosshealth.org]
> > Sent:	Monday, May 10, 1999 3:15 PM
> > To:	histonet@Pathology.swmed.edu
> > Subject:	CD15 antibody
> > 
> > Currently we are using DAKO predilutes on the DAKO immuno stainer with
> > WONDERFUL results.  However, as fate would have, our Hempath wants to
> > do CD15 using a non-clone.  DAKO only supplies the clone.  Does anyone
> > (using the DAKO) have a technique and a supplier for the CD15 antibody
> > that you would not mind sharing with us?
> > 
> > Better yet, is anyone using the Becton-Dickinson CD15 on the DAKO
> > stainer?
> > 
> > Edward Henry, Manager
> > Anatomic Pathology
> > Holy Cross Hospital
> > Silver Spring, MD
> > henrye@holycrosshealth.org