Re: Long reply Re: [Histonet] Heat & Hematoxylin

From:Joseph Saby



Another quick consideration:Most GLP labs require that the evvironment be controlled, and I would suspect the same for hospital labs. I could see temeprature variations causing major inconsistencies in special stains/histochemical reactions.Jose Saby, BS HT----- Original Message ----From: Gayle Callis To: Robyn Vazquez ; integrated.histo@gmail.com; histonet@lists.utsouthwestern.eduSent: Monday, May 19, 2008 3:32:32 PMSubject: Long reply Re: [Histonet] Heat & HematoxylinCindy and Robyn,We maintain a working temperature to approx 70 - 72F. Their suggested temperature range is outrageous. Your pathologists might find employees more productive with cooler working temperatures (you have to wear lab coats, gloves etc that add to discomfort). At the risk of being "catty", I presume your pathologists are on the "AC'd 7 am to 7 pm" schedule? What is the reasoning behind lowering temperature in that 12 hour period other than =0Asaving the almighty dollar. If the lab is that hot when the AC kicks in, isn't the system working harder (costing more) to cool down a lab? =0AShouldn't ALL employees have the same working conditions, including the =0Aearly morning crew. Fair is fair!I suggest you survey your chemicals and solvents, list the flash point and proper storage temperature for volatile, flammable and potentially explosive chemicals you have on hand. You safety can be compromised due to improper temperatures for chemical storage, usage/longevity. 85F to 92F is excessive. High temperature also affects how paraffin sections.Contact the hematoxylin manufacturer technical services, tell them of your plight and ask them if higher temperature is a problem. They put "controlled room temperature" on their containers for a very good reason but have them say why they put storage conditions on the bottle. Controlled room temperature, in one instance, had a range of 20 - 25C (68 - 77F). This range falls into what Wikipedia defined (see below). Heat accelerates chemical reactions, and probably contributes to the breakdown of chemical solutions too.The Wikipedia definition of Room Temperature was interesting:=0ARoom temperature (also referred to as ambient temperature) is a common term to denote a certain temperature within enclosed space at which humans are accustomed. Room temperature is thus often indicated by general human comfort, with the common range of 18C (64.4 F) to 24C (75.2 F), though climate may acclimatise people to higher or lower temperatures.The term can also refer to a temperature of food to be consumed (e.g., red wine) which is placed in such a room for a given time. Furthermore, it may refer to a certain temperature within settings of scientific experiments and calculations.For human comfort, desirable room temperature greatly depends on individual needs and various other factors. According to the West Midlands Public Health Observatory,[1] 21 C (69.8 F) is the recommended living room temperature, whereas 18 C (64.4 F) is the recommended bedroom temperature. A study carried out at the Uppsala University,[2] on indoor air quality and subjective indoor air quality (SIAQ) in primary schools, states that perception of high room temperature was related to a poor climate of cooperation. To achieve a good SIAQ, it recommends room temperature should be at a maximum of 24.0 C (75.5 F).Scientific definitionFor scientific calculations, room temperature is taken to be 20 to 23.5 degrees Celsius, 528 to 537 degrees Rankine (R), or 293 to 296 Kelvin (K), with an average of 21 C, about 70 degrees Fahrenheit (F).[3]. For numerical convenience, either 20 C or 300 K is often used. However, room temperature is not a precisely defined scientific term as opposed to Standard Temperature and Pressure, which has several, slightly different, definitions.=0AGood luck on solving this problem.Gayle M. CallisHTL/HT/MT(ASCP)Bozeman MT 59715----- Original Message ----- From: "Robyn Vazquez" To: ; Sent: Monday, May 19, 2008 12:08 PMSubject: Re: [Histonet] Heat & Hematoxylin> Cindy,> Do you have refrig/freezers, cryostats, and anything that puts out heat> from the motor? Our lab is between 68-70, our equipment doesn't have to=0A> work to stay at their temps. And the equipment will last longer. And> beside the tech have to be comfortable. It would be inconsiderate to> employees to have them suffer in a high temp such as 85 degrees. Does> the doctors have to stay in the lab constantly? Crank their offices heat> up to 85 and see how they like it, I bet not. Just my opinion.>> Robyn>>>>> "Cindy DuBois"  5/19/2008 7:54 AM >>>>> Our doctors are being very strict with the A/C.=A0 It only runs from 7am> to> 7pm. When we come into the lab in the morning @ 3:30 it is 82. This> morning I check the high for the weekend and it was 92 in the lab.> Within the last 2 weeks, the doctors are also complaining about our> washed-out dull looking hematoxylin. We have increased our staining> time to> 7 minutes (from 5) and decreased the acid alcohol to 1 dip. All the> label> says on the stain bottle is to keep the stain at "controlled room> temp". I> have mentioned this to the doctors but all I get is "85 can be> considered> room temp".> Does anyone have any specifications as to what temperature should be> maintained in a lab?> Cindy> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet>=0A>> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu=0Ahttp://lists.utsouthwestern.edu/mailman/listinfo/histonet      
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