We use xylene to remove coverslips and do this work inside a hood. After
coverslip is off and mounting media is gone with an extra xylene rinse, we
go back to Clearite 3 to rinse off xylene and recoverslip. Anything to try
an minimize exposure to xylene is done. Unfortunately, we found the
substitute just didn't work for this job.
Gayle M. Callis
----- Original Message -----
From: "Patty Dunlop"
Sent: Tuesday, May 20, 2008 1:20 PM
Subject: [Histonet] xylene substitute recoverslipping
> Hello Histonetters,
> I use Clear-Rite 3, a xylene substitute, and am having problems when I
> to recoverslip. Currently, I just put the slide that needs recoverslipped
> into a coplin jar of Clear-rite 3 at room temperature. The coverslip
> doesn't like to come off (takes a week) and it tends to be a sticky, messy
> process. Does anyone have a method for removing coverslips with a xylene
> substitute? Should I use heat?
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