Re: [Histonet] xylene substitute recoverslipping

From:"Gayle Callis"

We use xylene to remove coverslips and do this work inside a hood.  After 
coverslip is off and mounting media is gone with an extra xylene rinse, we 
go back to Clearite 3 to rinse off xylene and recoverslip.  Anything to try 
an minimize exposure to xylene is done.  Unfortunately, we found the 
substitute just didn't work for this job.

Gayle M. Callis
Bozeman MT

----- Original Message ----- 
From: "Patty Dunlop" 
Sent: Tuesday, May 20, 2008 1:20 PM
Subject: [Histonet] xylene substitute recoverslipping

> Hello Histonetters,
> I use Clear-Rite 3, a xylene substitute, and am having problems when I 
> need
> to recoverslip.  Currently, I just put the slide that needs recoverslipped
> into a coplin jar of Clear-rite 3 at room temperature.  The coverslip 
> really
> doesn't like to come off (takes a week) and it tends to be a sticky, messy
> process.  Does anyone have a method for removing coverslips with a xylene
> substitute?  Should I use heat?
> Thanks,
> Patty
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