You have limited the options because you are talking about "overnight".
I also strongly recommend you to stop that practice of putting the biopsies in water after being fixed. That is no good procedure. If you have a problem with the fumes, place all the cassettes with formalin in a fumes hood.
Pressure - vacuum in the tissue processor is to assure better exchange of reagents and it should be used if your tissue processor has that capability (as all VIPs do).
You can start in 80% EthOL and if the biopsies have fat you should increase the xylene times.
A 4 hours protocol will be more than enough.
Try that the end of the processing time coincides with the staring time for embedding, which means that the specimens should be in formalin (first station) until the process starts.
KELLY BOYD wrote:
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